| 检测B型葡萄球菌肠毒素的Love波免疫传感器研究 | |
| 刘虹 ; 高志贤 ; 王源升 ; 刘清山 ; 朱惠忠 ; LIU Hong ; GAO Zhi-xian ; WANG Yuan-sheng ; LIU Qing-shan ; ZHU Huizhong | |
| 2010-06-08 ; 2010-06-08 | |
| 关键词 | Love声表面波免疫传感器 B型葡萄球菌肠毒素 蛋白A Love mode SAW immunosensor staphylococcal enterotoxins B(SEB) protein A TP212 |
| 其他题名 | Study on Love mode SAW immunosensor for staphylococcal enterotoxins B |
| 中文摘要 | 报道了一种可用于检测B型葡萄球菌肠毒素(SEB)的Love模式声表面波压电免疫传感器。传感器本身由压电石英基片、叉指换能器和S iO2声波导层构成。通过APTES和戊二醛层交联蛋白A,实现抗体分子的定向固定,在Love波传感器的表面构建出抗体分子探针的传感界面。建立了基于网络分析仪的传感器测试系统,对抗体分子固定化过程以及10-6g/mL SEB的响应过程进行了实时监测,相位的偏移随时间表现出明显而规律性的改变,与戊二醛共价交联法进行了对比,并用原子力显微镜(AFM)对蛋白A法固定抗体以及捕获了SEB后的表面进行了成像表征。; A Love mode surface acoustic waves(SAW) immunosensor for staphylococcal enterotoxins B(SEB) was presented.The Love wave devices with the substrate of ST-cut quartz,interdigital transducers(IDTs) and the guiding layers of SiO_2 were fabricated successfully.The active area of sensor was precoated with γ-aminopropylethoxysilane(APTES) and glutaraldehyde,then antibody molecule probes were immobilized in a highly oriented manner by the protein A on SiO_2 surface.A system based on network analyzer was set up for conducting the immunosensing experiments.In these experiments,immobilization of antibody onto the active area of the sensor and binding with 10~(-6) g/mL SEB were monitored,the phase shift of the sensor system changed obviously.The surface morphology of the active area were further characterized by using atomic force microscopy(AFM) after protein A-based immobilization of antibodies and binding with 10~(-6) g/mL SEB. |
| 语种 | 中文 ; 中文 |
| 内容类型 | 期刊论文 |
| 源URL | [http://hdl.handle.net/123456789/49040]  |
| 专题 | 清华大学 |
| 推荐引用方式 GB/T 7714 | 刘虹,高志贤,王源升,等. 检测B型葡萄球菌肠毒素的Love波免疫传感器研究[J],2010, 2010. |
| APA | 刘虹.,高志贤.,王源升.,刘清山.,朱惠忠.,...&ZHU Huizhong.(2010).检测B型葡萄球菌肠毒素的Love波免疫传感器研究.. |
| MLA | 刘虹,et al."检测B型葡萄球菌肠毒素的Love波免疫传感器研究".(2010). |
| 个性服务 |
| 查看访问统计 |
| 相关权益政策 |
| 暂无数据 |
| 收藏/分享 |
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论