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	CaCl_2作为异硫氰酸曙红固体基质室温磷光增强剂及其在免疫分析中的应用
	吴娅兰 ; 李隆弟 ; 刘佳铭 ; 朱国辉 ; WU Ya-Lan ; LI Long-Di ; LIU Jia-Ming ; ZHU Guo-Hui
	2010-06-07 ; 2010-06-07
关键词	固体基质室温磷光免疫分析法 异硫氰酸曙红 CaCl2作增强剂 人IgG Solid substrate room temperature phosphorescence immunoassay Eosin-5-isothiocynate CaCl 2 as an enhancer IgG O657.3
其他题名	Solid Substrate Room Temperature Phosphorescence of Eosin-5-isothiocynate and Application in Immune Analysis Using CaCl_2 as an Enhancer
中文摘要	Room temperature phosphorescence(RTP) signal intensity of eosin-ITC on a polyamide membrane(PM) was very low, but the signal was increased obviously once some inorganic salts were added into the system at λ ex/λ em=532 nm/685 nm. The enhancement effect of ⅡA element ions(such as Ca 2+, Sr 2+ and Ba 2+) on RTP of eosin-ITC is much obvious than that of Pb 2+ and Hg 2+ used constantly as an outer heavy atom perturber in phosphorimetry. Based on that, a new solid substrate room temperature phosphorescence immunoassay(SS-RTP-IA) for the determination of the human IgG was proposed to demonstrate the feasibility using eosin-ITC as phosphorescent marker. It was found that a good linear relationship with the ΔI P was obtained when the amount of human IgG in the range from 6.25 to 400 pg/spot(corresponding to the mass concentration: 15.63 ng/mL—1 μg/L, sample volume of 0.4 μL/spot). The regression {equation} of working curve can be expressed as ΔI p=3.062+0.00864x(pg)(n=7, r=0.996), The detection limit calculated as 3S b/k is 0.394 pg/spot. The method was applied directly to the determination of IgG in human serum with a satisfying result. These results show that eosin-ITC can be used conveniently as a RTP marker of high sensitivity.; 国家自然科学基金 (批准号 :2 0 175 0 11); 福建省自然科学基金 (批准号 :C0 110 0 2 8)资助
语种	中文 ; 中文
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/40646]
专题	清华大学
推荐引用方式 GB/T 7714	吴娅兰,李隆弟,刘佳铭,等. CaCl_2作为异硫氰酸曙红固体基质室温磷光增强剂及其在免疫分析中的应用[J],2010, 2010.
APA	吴娅兰.,李隆弟.,刘佳铭.,朱国辉.,WU Ya-Lan.,...&ZHU Guo-Hui.(2010).CaCl_2作为异硫氰酸曙红固体基质室温磷光增强剂及其在免疫分析中的应用..
MLA	吴娅兰,et al."CaCl_2作为异硫氰酸曙红固体基质室温磷光增强剂及其在免疫分析中的应用".(2010).

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