反相高效液相色谱法同时测定姜黄药材中3个组分的含量 | |
刘硕谦 ; 刘仲华 ; 黄建安 ; 田娜 ; 陆英 ; 罗国安 ; Liu Shuoqian ; Liu Zhonghua ; Huang Jianan ; Tian Na ; Lu Ying ; Luo Guoan | |
2010-06-07 ; 2010-06-07 | |
关键词 | 反相高效液相色谱 姜黄 姜黄素类化合物 Reversed-phase high performance liquid chromatography, Curcuma longa L., curcuminoids TQ461 O657.7 |
其他题名 | Simultaneous Determination of Three Main Components in Curcuma longa L. by Reversed-phase High Performance Liquid Chromatography |
中文摘要 | 建立了一种反相高效液相色谱方法,同时测定中药材姜黄块根中3组分的含量。采用KromasilTMC18柱(5μm,200mm×4.6mm),以磷酸水溶液(pH=2.5)和乙腈溶剂为流动相,梯度洗脱,流速0.9mL/min,柱温40℃,检测波长为425nm,10min内即可将3种组分分离测定。该方法快速、简便、准确,可为评价不同产地的姜黄质量提供依据。; A reversed-phase high performance liquid chromatographic method was established for simultaneous determination of curcumin, demethoxycurcumin and bidemethoxycnrcumin in the rhizome of Curcuma longa L., a typical herbaceous plant. A Kromasil~TM C_~18 column was used with a mixture of acetonitrile and phosphoric acid aqueous solution (pH=2.5) as the mobile phase. The flow rate was 0.9 mL/min and the detection wavelength was set at 425 nm. The three constituents were separated within 10 min. The method is rapid, simple and ~accurate , and it can be used for the evaluation of Curcuma longa L.; 科技部技术创新基金资助项目(No.01C26124300713) |
语种 | 中文 ; 中文 |
内容类型 | 期刊论文 |
源URL | [http://hdl.handle.net/123456789/40573] |
专题 | 清华大学 |
推荐引用方式 GB/T 7714 | 刘硕谦,刘仲华,黄建安,等. 反相高效液相色谱法同时测定姜黄药材中3个组分的含量[J],2010, 2010. |
APA | 刘硕谦.,刘仲华.,黄建安.,田娜.,陆英.,...&Luo Guoan.(2010).反相高效液相色谱法同时测定姜黄药材中3个组分的含量.. |
MLA | 刘硕谦,et al."反相高效液相色谱法同时测定姜黄药材中3个组分的含量".(2010). |
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