结合丝组二肽的树状多肽对λDNA的切割 | |
何骏 ; 麻远 ; 赵玉芬 ; HE Jun ; MA Yuan ; ZHAO Yu-Fen | |
2010-06-07 ; 2010-06-07 | |
关键词 | 丝组二肽 树状多肽 DNA切割 Seryl-histdine dipeptide Peptide dendrimer DNA cleavage Q523 |
其他题名 | Cleavage of λDNA by Seryl-histidine Planted Peptide Dendrimer |
中文摘要 | Artificial nucleic acid cleavage agents have received considerable attention due to their very important applications in biochemistry and molecular biology.Seryl-histidine is a dipeptide possessing DNA,protein and ester cleavage activity.Since peptide dendrimers have a lot of unique physical and chemical properties compared to normal peptide chain and have a great varieties of applications in biological areas,two and four branched seryl-histidine planted peptide dendrimers were synthesized by Fmoc solid phase peptide synthesis approach.The products were purified by HPLC and characterized by ESI-MS.Their DNA cleavage activities were tested by means of Agarose Gel Electrophoresis.In the cleavage reaction,peptide dendrimer showed a strong combining inclination to DNA,highly concentrated dendrimer solution can quench the fluorescence of EB.Even though,the DNA cleavage activity of the dendrimers was lower than that of seryl-histidine dipeptide monomer.; 教育部留学回国人员科研启动基金; 国家自然科学基金(批准号:20132020)资助. |
语种 | 中文 ; 中文 |
内容类型 | 期刊论文 |
源URL | [http://hdl.handle.net/123456789/40511] ![]() |
专题 | 清华大学 |
推荐引用方式 GB/T 7714 | 何骏,麻远,赵玉芬,等. 结合丝组二肽的树状多肽对λDNA的切割[J],2010, 2010. |
APA | 何骏,麻远,赵玉芬,HE Jun,MA Yuan,&ZHAO Yu-Fen.(2010).结合丝组二肽的树状多肽对λDNA的切割.. |
MLA | 何骏,et al."结合丝组二肽的树状多肽对λDNA的切割".(2010). |
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