QQ客服 官方微博 反馈留言

	结合丝组二肽的树状多肽对λDNA的切割
	何骏 ; 麻远 ; 赵玉芬 ; HE Jun ; MA Yuan ; ZHAO Yu-Fen
	2010-06-07 ; 2010-06-07
关键词	丝组二肽 树状多肽 DNA切割 Seryl-histdine dipeptide Peptide dendrimer DNA cleavage Q523
其他题名	Cleavage of λDNA by Seryl-histidine Planted Peptide Dendrimer
中文摘要	Artificial nucleic acid cleavage agents have received considerable attention due to their very important applications in biochemistry and molecular biology.Seryl-histidine is a dipeptide possessing DNA,protein and ester cleavage activity.Since peptide dendrimers have a lot of unique physical and chemical properties compared to normal peptide chain and have a great varieties of applications in biological areas,two and four branched seryl-histidine planted peptide dendrimers were synthesized by Fmoc solid phase peptide synthesis approach.The products were purified by HPLC and characterized by ESI-MS.Their DNA cleavage activities were tested by means of Agarose Gel Electrophoresis.In the cleavage reaction,peptide dendrimer showed a strong combining inclination to DNA,highly concentrated dendrimer solution can quench the fluorescence of EB.Even though,the DNA cleavage activity of the dendrimers was lower than that of seryl-histidine dipeptide monomer.; 教育部留学回国人员科研启动基金; 国家自然科学基金(批准号:20132020)资助.
语种	中文 ; 中文
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/40511]
专题	清华大学
推荐引用方式 GB/T 7714	何骏,麻远,赵玉芬,等. 结合丝组二肽的树状多肽对λDNA的切割[J],2010, 2010.
APA	何骏,麻远,赵玉芬,HE Jun,MA Yuan,&ZHAO Yu-Fen.(2010).结合丝组二肽的树状多肽对λDNA的切割..
MLA	何骏,et al."结合丝组二肽的树状多肽对λDNA的切割".(2010).

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。