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	中国小型猪骨髓间充质干细胞的蛋白质组研究
	叶能胜 ; 张荣利 ; 罗国安 ; 冯雪 ; 赵艳峰 ; 王义明 ; Ye Nengsheng ; Zhang Rongli ; Luo Guoan ; Feng Xue ; Zhao Yanfeng ; Wang Yiming
	2010-06-07 ; 2010-06-07
关键词	骨髓间充质干细胞 蛋白质组 二维电泳 质谱 Bone marrow mesenchymal stem cell,proteome,twodimensional gel electrophoresis,mass spectrometry Q952
其他题名	Proteome Analysis of Chinese Miniswine Bone Marrow Mesenchymal Stem Cells
中文摘要	报道了骨髓间充质干细胞(MSCs)的蛋白质组表达研究。从体外培养的MSCs提取细胞蛋白,经二维电泳分离后用银染方法可检出蛋白点约1600个,选取48个蛋白点进行胶内酶解及质谱分析,经数据库检索成功鉴定了37个蛋白,并对蛋白功能进行初步分析。本实验数据为进一步分析MSCs增殖、分化或凋亡的分子机理提供相关信息。; The proteome of Chinese miniswine bone marrow mesenchymal stem cells(MSCs) was investigated.Proteins extracted from MSCs were separated by two-dimensional gel electrophoresis(2DE),and about 1600 protein spots were detected by the silver nitrate staining method.48 proteins were(digested) by trypsin for mass spectrometric analysis.37 proteins were successfully identified by peptide mass fingerprinting(PMF).The biological functions of these identified proteins were involved in different cell processes. Proteomics was successfully applied for the protein expression analysis of Chinese miniswine bone marrow MSCs and some(useful) data were provided for the future study of MSCs.; 国家自然科学基金资助项目(No.30271663、90209005、90209035)
语种	中文 ; 中文
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/40491]
专题	清华大学
推荐引用方式 GB/T 7714	叶能胜,张荣利,罗国安,等. 中国小型猪骨髓间充质干细胞的蛋白质组研究[J],2010, 2010.
APA	叶能胜.,张荣利.,罗国安.,冯雪.,赵艳峰.,...&Wang Yiming.(2010).中国小型猪骨髓间充质干细胞的蛋白质组研究..
MLA	叶能胜,et al."中国小型猪骨髓间充质干细胞的蛋白质组研究".(2010).

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