Quasi-3D Cytoskeletal Dynamics of Osteocytes under Fluid Flow

	Quasi-3D Cytoskeletal Dynamics of Osteocytes under Fluid Flow
	Baik AD; Lu XL; Qiu J; Huo B(霍波); Hillman EMC; Dong C; Guo XE; Huo B
刊名	Biophysical Journal
	2010
卷号	99 期号:9 页码:2812-2820
关键词	Living Endothelial-Cells Viscoelastic Properties Articular-Cartilage Shear-Stress Bone-Cells In-Vitro Adhesion Deformation Prestress Strain
ISSN号	0006-3495
通讯作者	Huo B
中文摘要	Osteocytes respond to dynamic fluid shear loading by activating various biochemical pathways, mediating a dynamic process of bone formation and resorption. Whole-cell deformation and regional deformation of the cytoskeleton may be able to directly regulate this process. Attempts to image cellular deformation by conventional microscopy techniques have been hindered by low temporal or spatial resolution. In this study, we developed a quasi-three-dimensional microscopy technique that enabled us to simultaneously visualize an osteocyte's traditional bottom-view profile and a side-view profile at high temporal resolution. Quantitative analysis of the plasma membrane and either the intracellular actin or microtubule (MT) cytoskeletal networks provided characterization of their deformations over time. Although no volumetric dilatation of the whole cell was observed under flow, both the actin and MT networks experienced primarily tensile strains in all measured strain components. Regional heterogeneity in the strain field of normal strains was observed in the actin networks, especially in the leading edge to flow, but not in the MT networks. In contrast, side-view shear strains exhibited similar subcellular distribution patterns in both networks. Disruption of MT networks caused actin normal strains to decrease, whereas actin disruption had little effect on the MT network strains, highlighting the networks' mechanical interactions in osteocytes.
类目[WOS]	Biophysics
研究领域[WOS]	Biophysics
关键词[WOS]	LIVING ENDOTHELIAL-CELLS ; VISCOELASTIC PROPERTIES ; ARTICULAR-CARTILAGE ; SHEAR-STRESS ; BONE-CELLS ; IN-VITRO ; ADHESION ; DEFORMATION ; PRESTRESS ; STRAIN
收录类别	SCI
资助信息	National Institutes of Health [AR058453, AR052417]
原文出处	http://dx.doi.org/10.1016/j.bpj.2010.08.064
语种	英语
WOS记录号	WOS:000283912600015
公开日期	2011-03-01 ; 2011-03-30
内容类型	期刊论文
源URL	[http://dspace.imech.ac.cn/handle/311007/43623]
专题	力学研究所_国家微重力实验室
通讯作者	Huo B
推荐引用方式 GB/T 7714	Baik AD,Lu XL,Qiu J,et al. Quasi-3D Cytoskeletal Dynamics of Osteocytes under Fluid Flow[J]. Biophysical Journal,2010,99(9):2812-2820.
APA	Baik AD.,Lu XL.,Qiu J.,霍波.,Hillman EMC.,...&Huo B.(2010).Quasi-3D Cytoskeletal Dynamics of Osteocytes under Fluid Flow.Biophysical Journal,99(9),2812-2820.
MLA	Baik AD,et al."Quasi-3D Cytoskeletal Dynamics of Osteocytes under Fluid Flow".Biophysical Journal 99.9(2010):2812-2820.