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	感冒一小时胶囊中黄芩苷及盐酸小檗碱的HPLC测定
	石钺 ; 李开通 ; 杨帅 ; 杜力军 ; SHI Yue ; LI Kai-tong ; YANG Shuai ; DU Li-jun
	2010-05-14 ; 2010-05-14
关键词	HPLC 感冒一小时胶囊 黄芩苷 盐酸小檗碱 HPLC Ganmao Yixiaoshi Capsule baicalin berberine R286.0
其他题名	Determination of baicalin and berberine in Ganmao Yixiaoshi Capsule by HPLC
中文摘要	目的:建立高效液相色谱法对感冒一小时胶囊(羌活、独活、粉葛、黄芩、黄莲等)中黄芩苷和盐酸小檗碱测定方法。方法:用Hypersil C18(4.6mm×150mm,5μm)为色谱柱;分别以乙腈-0.05mol/L磷酸水溶液(25∶75)和乙腈-0.05mol/L磷酸二氢钾缓冲液(含0.5%三乙胺,磷酸调pH3.0)(24∶76)为流动相,检测波长分别为276nm和346nm。结果:黄芩苷线性范围0.176μg~0.880μg,相关系数为0.9998,盐酸小檗碱线性范围0.148μg~0.845μg,相关系数为0.9995;平均回收率黄芩苷99.30%(RSD=1.56%),盐酸小檗碱98.84%(RSD=2.41%)。结论:方法简便、准确、灵敏可靠,为感冒一小时胶囊的质量控制提供了依据。; AIM:To establish methods for determining baicalin and berberine in Ganmao Yixiaoshi Capsule(Rhizoma et Radix Notopterygii,Radix Angelicae Pubescentis,Radix Puerariae thomsonii,Radix Scutellariae,Rhizoma Coptidis,etc.)by HPLC.METHODS:Hypersil C_ 18(4.6 mm×150 mm,5 μm)column was used,the mobile phase was composed of acetonitrile-0.05 mol/L phosphoric acid(25 ∶75)to determine baicalin,acetonitrile-0.05 mol/L potassium dihydrogen phosphate buffer solution(with 0.3% triethylamine,pH 3.0 adjusted with phosphoric acid)(24 ∶76)to determine berberine.The detection wavelengths were at 276 nm and 346 nm,respectively.RESULTS:The calibration curve was linear in the concentration ranges of 0.176 μg-0.880 μg for baicalin and 0.148 μg-0.845 μg for berberine,the correlation coefficient were 0.999 8 and 0.999 5,respectively.The average recovery of baicalin was 99.30%(RSD= 1.56%)and that of berberine was 98.84%(RSD= 2.41%).CONCLUSION:The method is sensitive,simple and accurate,and can be used for the quality control of Ganmao Yixiaoshi Capsule.
语种	中文 ; 中文
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/33897]
专题	清华大学
推荐引用方式 GB/T 7714	石钺,李开通,杨帅,等. 感冒一小时胶囊中黄芩苷及盐酸小檗碱的HPLC测定[J],2010, 2010.
APA	石钺.,李开通.,杨帅.,杜力军.,SHI Yue.,...&DU Li-jun.(2010).感冒一小时胶囊中黄芩苷及盐酸小檗碱的HPLC测定..
MLA	石钺,et al."感冒一小时胶囊中黄芩苷及盐酸小檗碱的HPLC测定".(2010).

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