| 硝基苯胺单克隆抗体的制备和效果评价 | |
| 彭方毅 ; 何苗 ; 宋保栋 ; 姜海蓉 ; 盛建武 ; 施汉昌 ; PENG Fangyi ; HE Miao ; SONG Baodong ; JIANG Hairong ; SHENG Jianwu ; SHI Hanchang | |
| 2010-05-13 ; 2010-05-13 | |
| 关键词 | 环境监测 酶联免疫分析法(ELISA) 硝基苯胺 单克隆抗体 environmental monitoring enzyme linked immuno-sorbent assay(ELISA) nitroaniline monoclonal antibody X832 |
| 其他题名 | Preparation and evaluation of monoclonal antibody against nitroaniline |
| 中文摘要 | 为了测定水中硝基苯胺类化合物,进而开发酶联免疫分析法(EL ISA)快速检测方法,制备了抗硝基苯胺单克隆抗体。以合成的硝基苯胺完全抗原,作为免疫原免疫B a lb/c小鼠,采用B细胞杂交瘤技术,经免疫、融合、筛选和克隆等,得到了抗硝基苯胺单克隆抗体。该抗体亚类为IgG1,制备的单克隆抗体腹水效果评价达10-7,与其他硝基苯胺结构类似物无交叉反应。实验结果表明,用本间接竞争EL ISA方法可以精确地检测不同水样中硝基苯胺残留。; A monoclonal antibody(McAb) against nitroaniline was prepared for measuring nitroaniline residuals and for an enzyme linked immuno-sorbent assay(ELISA) reagent kit.Balb/c mice were immunized intraperitoneally with nitroaniline coupled with bovine serum albumin.Hybridoma cell lines secreting McAbs were obtained using routine hybridoma technology and ELISA screening.The McAbs subtype was IgG1.The ascite titer was 10-7.The McAbs had no cross-reaction with some analogues of nitroaniline.The results show that the indirect competitive ELISA can be used to accurately detect nitroaniline residues in various water samples and be benefit for environmental monitoring. |
| 语种 | 中文 ; 中文 |
| 内容类型 | 期刊论文 |
| 源URL | [http://hdl.handle.net/123456789/30638]  |
| 专题 | 清华大学 |
| 推荐引用方式 GB/T 7714 | 彭方毅,何苗,宋保栋,等. 硝基苯胺单克隆抗体的制备和效果评价[J],2010, 2010. |
| APA | 彭方毅.,何苗.,宋保栋.,姜海蓉.,盛建武.,...&SHI Hanchang.(2010).硝基苯胺单克隆抗体的制备和效果评价.. |
| MLA | 彭方毅,et al."硝基苯胺单克隆抗体的制备和效果评价".(2010). |
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