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	组织因子小干扰RNA壳聚糖纳米粒的制备及其特征
	邱雪峰 ; 董念国 ; 孙宗全 ; 胡平 ; 史嘉玮 ; 陈思 ; Qiu Xuefeng ; Dong Nianguo ; Sun Zongquan
	2010-05-13 ; 2010-05-13
关键词	RNA干扰 纳米粒 壳聚糖 组织因子 RNA interference nanoparticles chitosan tissue factor R318.08
其他题名	Preparation and Characteristics of Chitosan Nanoparticles for Tissue Factor siRNA Delivery
中文摘要	目的探讨壳聚糖-组织因子小干扰RNA(TFsiRNA)纳米粒制备方法并研究其特性。方法采用三聚磷酸钠(TPP)离子胶凝法分别制备包封型和吸附型壳聚糖-TPP-TFsiRNA纳米粒。检测纳米粒平均粒径和Zeta电位、载药率,并分析血清稳定性、体外生物活性及其细胞毒性。结果两种纳米粒粒径均小于550nm,粒径大小主要取决于壳聚糖类型、分子量及其浓度。酸碱度、壳聚糖TPP质量比也影响粒径大小。包封型纳米粒siRNA载药率为100%。壳聚糖-TPP-TFsiRNA纳米粒中的siRNA在5%血清中孵育24h开始降解,60h完全降解;50%血清孵育6h保持完整,48h完全降解。结论壳聚糖纳米粒可能成为有效的siRNA转运载体。; Objective To prepare a novel chitosan-based tissue factor siRNA nanoparticle delivery system and investigate the characteristics.Methods Chitosan nanoparticles were prepared by ionic gelation using sodium tripolyphosphate.Mean particle diameter,Zeta potential and siRNA loading efficiency of the nanoparticles were determined.Serum stability and biological activity of chitosan-siRNA nanoparticles were assayed.The effect of chitosan on cytotoxicity was measured by determining cell viability of chitosan-siRNA nanoparticles.Results Both methods produced nanosize particles,less than 550 nm dependent on type,molecular weight as well as concentration of chitosan.In the case of ionic gelation,two further factors,namely chitosan to TPP weight ratio and pH,affected the particle size.100% siRNA loading efficiency was obtained.siRNA recovered from chitosan-TPP nanoparticles started to degrade after 24 h incubation and full degradation was only observed after 60 h incubation in 5% serum,and siRNA recovered from chitosan-siRNA nanoparticles was intact up to 6 h and fully degraded after 48 h incubation in 50% serum.Conclusion Chitosan-TPP nanoparticles show much potential as viable vector candidates for cost-effective siRNA delivery.
语种	中文 ; 中文
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/30209]
专题	清华大学
推荐引用方式 GB/T 7714	邱雪峰,董念国,孙宗全,等. 组织因子小干扰RNA壳聚糖纳米粒的制备及其特征[J],2010, 2010.
APA	邱雪峰.,董念国.,孙宗全.,胡平.,史嘉玮.,...&Sun Zongquan.(2010).组织因子小干扰RNA壳聚糖纳米粒的制备及其特征..
MLA	邱雪峰,et al."组织因子小干扰RNA壳聚糖纳米粒的制备及其特征".(2010).

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