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	Study on the non-covalent interaction of 7-hydroxy flavone and its phosphate with DNA by fluorescence method
	Guo, YH ; Chen, XL ; Zhang, T ; Qu, LB ; Zhao, YF ; Yu, YZ
	2010-05-11 ; 2010-05-11
关键词	EB probe DNA 7-hydroxy flavone diethyl flavon-7-yl phosphate fluorescence quenching non-covalent interaction METAL-COMPLEXES BINDING CHRYSIN Spectroscopy
中文摘要	The non-covalent interaction of 7-hydroxy flavone and its phosphate with DNA was studied using ethidium bromide (EB)as a probe. The result showed that both 7-hydroxy flavone and its phosphate could form non-covalent complexes, but the phosphorylated flavonoid showed higher binding affinity with DNA than 7-hydroflavone did. Experiments demonstrated that the higher the temperature, the lower the slop of quenching curve of DNA-EB in the presence of different amounts of 7-hydroxy flavone and its phosphate. It was confirmed that the combinations of DNA with 7-hydroxy flavone and its phosphate were a single static quenching process. According to the Stern-Volmer equation and Scatchard equation the quenching constants and the intrinsic binding constants of 7-hydroxy flavone and its phosphate were measured respectively, they were K-q1 = 601 L center dot mol(-1), K-q2 = 1 381 L center dot mol(-1); K-1 =2.07x10(4) L center dot mol(-1), K-2 =3.19x10(4) L center dot mol(-1) respectively.
语种	中文 ; 中文
出版者	BEIJING UNIV PRESS ; BEIJING ; HAIDIAN-QU, BEIJING 100871, PEOPLES R CHINA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/27668]
专题	清华大学
推荐引用方式 GB/T 7714	Guo, YH,Chen, XL,Zhang, T,et al. Study on the non-covalent interaction of 7-hydroxy flavone and its phosphate with DNA by fluorescence method[J],2010, 2010.
APA	Guo, YH,Chen, XL,Zhang, T,Qu, LB,Zhao, YF,&Yu, YZ.(2010).Study on the non-covalent interaction of 7-hydroxy flavone and its phosphate with DNA by fluorescence method..
MLA	Guo, YH,et al."Study on the non-covalent interaction of 7-hydroxy flavone and its phosphate with DNA by fluorescence method".(2010).

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