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	Determination of ddehydroepiandrosterone in emasculated rat's serum by gas chromatography-tandem mass spectrometry
	Jup, Chen ; Liang Qiong-Lin ; Luo Guo-An ; Wang Yi-Ming ; Duan Xue-Yan ; Zhang Hong-Yang
	2010-05-11 ; 2010-05-11
关键词	dehydroepiandrosterone gas chromatography-tandem mass spectrometry adrenal androgen clinical analysis PROSTATE-CANCER DEHYDROEPIANDROSTERONE DHEA THERAPY TISSUE MICE Chemistry, Analytical
中文摘要	Dehydroepiandrosterone DHEA which is mostly secreted from adrenal in prostate cancer patients with anti-androgen therapy, is considered as the very inducer who should take responsibility for the androgen-independent transition. DHEA serum concentration in Rat with orchiectomy for different time was detected by a gas chromatography/electron impact ionization tandem mass spectrometric method, which had been developed for the determination of DHEA in serum samples. Methanol was used to deproteinize the serum sample, then DHEA was analyzed by GC-MS/MS after SPE process and derivatization by heptafluorobutyric anhydride (HFBA). Sample pretreatment and GC-MS/MS analytical condition were optimized. The linear range was 0.1 - 100 mu g/L. The correlation coefficient (r) was 0.9996. The average recovery of DHEA in serum sample (from 2 mu g/L to 50 mu g/L) ranged from 99% to 106%, and the relative standard deviation was less than 5%. The result indicats that the method developed is more sensitive and selective. It also demonstrated that this analysis method could meet the requirement for the clinical determination of DHEA in serum samples. The result shows that there really exists a raise of DHEA in rat's serum after orchiectomy.
语种	中文 ; 中文
出版者	SCIENCE CHINA PRESS ; BEIJING ; 16 DONGHUANGCHENGGEN NORTH ST, BEIJING 100717, PEOPLES R CHINA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/27580]
专题	清华大学
推荐引用方式 GB/T 7714	Jup, Chen,Liang Qiong-Lin,Luo Guo-An,et al. Determination of ddehydroepiandrosterone in emasculated rat's serum by gas chromatography-tandem mass spectrometry[J],2010, 2010.
APA	Jup, Chen,Liang Qiong-Lin,Luo Guo-An,Wang Yi-Ming,Duan Xue-Yan,&Zhang Hong-Yang.(2010).Determination of ddehydroepiandrosterone in emasculated rat's serum by gas chromatography-tandem mass spectrometry..
MLA	Jup, Chen,et al."Determination of ddehydroepiandrosterone in emasculated rat's serum by gas chromatography-tandem mass spectrometry".(2010).

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