QQ客服 官方微博 反馈留言

	Development of a base stacking hybridization-based microarray method for rapid identification of clinical isolates
	Zhu, Ling-Xiang ; Wang, Dong ; Zhang, Guan-Bin ; Jiang, Di ; Zhang, Zhi-Wei ; Zhang, Qiong ; Mitchelson, Keith ; Cheng, Jing
	2010-05-11 ; 2010-05-11 ; OCT
关键词	base stacking hybridization (BSH) oligonucleotide microarray ribosomal RNA (rRNA) clinical isolates species identification 16S RIBOSOMAL-RNA ESCHERICHIA-COLI OLIGODEOXYRIBONUCLEOTIDE MICROCHIPS OLIGONUCLEOTIDE MICROARRAYS THERMODYNAMIC ANALYSIS STAPHYLOCOCCUS-AUREUS BLOOD CULTURES BACTERIA DNA CHIPS Infectious Diseases Microbiology
中文摘要	A base stacking hybridization-based microarray method was developed for rapid identification of clinical isolates within 2 It. The oligonucleotide probe sequences for species or genus-level identification were targeted against ribosomal RNA. Isolates were lysed and directly hybridized to the microarray-bound capture probes without conventional DNA or RNA isolation and prior polymerase chain reaction amplification. Five bacterial species encountered frequently in the clinical setting, Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae and Enterobacter cloacae, and one genus Enterococcus, could be discriminated by the microarray-based assay. Identification by this method matched biochemical identification for 150 of 152 clinical strains. This base-stacking hybridization microarray offers a simple, fast (<= 2 h), and accurate identification of bacterial cultures and is a potential tool for routine clinical diagnosis. (C) 2007 Elsevier Inc. All rights reserved.
语种	英语 ; 英语
出版者	ELSEVIER SCIENCE INC ; NEW YORK ; 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/27553]
专题	清华大学
推荐引用方式 GB/T 7714	Zhu, Ling-Xiang,Wang, Dong,Zhang, Guan-Bin,et al. Development of a base stacking hybridization-based microarray method for rapid identification of clinical isolates[J],2010, 2010, OCT.
APA	Zhu, Ling-Xiang.,Wang, Dong.,Zhang, Guan-Bin.,Jiang, Di.,Zhang, Zhi-Wei.,...&Cheng, Jing.(2010).Development of a base stacking hybridization-based microarray method for rapid identification of clinical isolates..
MLA	Zhu, Ling-Xiang,et al."Development of a base stacking hybridization-based microarray method for rapid identification of clinical isolates".(2010).

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。