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	Characterization of a thermostable alkaline phosphatase from a novel species Thermus yunnanensis sp nov and investigation of its cobalt activation at high temperature
	Gong, NP ; Chen, CY ; Xie, LP ; Chen, HT ; Lin, XZ ; Zhang, RQ
	2010-05-11 ; 2010-05-11
关键词	thermostable alkaline phosphatase Thermus yunnanensis sp nov. thermostability cobalt activation CALDOPHILUS GK24 PURIFICATION ENZYME MECHANISM PROTEINS ELISA GENE Biochemistry & Molecular Biology Biophysics
中文摘要	A thermostable alkaline phosphatase with high specific activity and thermal resistance was purified from a novel species of Thermus sp. named as Thermus yunnanensis sp. nov. The enzyme contains a single peptide with a molecular mass of about 52 kDa on SDS-PAGE analysis and appears to be a homodimer in solution with the molecular mass of 104 kDa. The optimal pH and temperature for its activities are pH 8.0-10.0 and 70-80 degrees C, respectively. The catalytic activities of the enzyme are metal ion dependent, and Mg2+, Zn2+ and Co2+ are the main activators. Among these, Co2+ is the most active stimulator and has unique activation effect at high temperature. Metal binding analysis showed the binding of Mg2+ at the metal binding site was easy to loss in the thermoinactivation, and Co2+ was apt to bind at that site and kept the favorable configuration of catalysis, which would result high activation in the incubation with Co2+ at high temperature. According to this study, a model was proposed for the explanation of the activation and the results of actual experiments demonstrated the validity of the model. (c) 2005 Elsevier B.V. All rights reserved.
语种	英语 ; 英语
出版者	ELSEVIER SCIENCE BV ; AMSTERDAM ; PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/27052]
专题	清华大学
推荐引用方式 GB/T 7714	Gong, NP,Chen, CY,Xie, LP,et al. Characterization of a thermostable alkaline phosphatase from a novel species Thermus yunnanensis sp nov and investigation of its cobalt activation at high temperature[J],2010, 2010.
APA	Gong, NP,Chen, CY,Xie, LP,Chen, HT,Lin, XZ,&Zhang, RQ.(2010).Characterization of a thermostable alkaline phosphatase from a novel species Thermus yunnanensis sp nov and investigation of its cobalt activation at high temperature..
MLA	Gong, NP,et al."Characterization of a thermostable alkaline phosphatase from a novel species Thermus yunnanensis sp nov and investigation of its cobalt activation at high temperature".(2010).

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