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A chromatographic method for baicalin quantification in rat thalamus
Zhang, LJ ; Xing, DM ; Ding, Y ; Wang, RF ; Wang, XL ; Du, LJ
2010-05-11 ; 2010-05-11
关键词baicalin intravenous administration rat thalamus reversed-phase HPLC SCUTELLARIA-BAICALENSIS FLAVONOIDS Biochemical Research Methods Biochemistry & Molecular Biology Chemistry, Analytical Pharmacology & Pharmacy
中文摘要A rapid reversed-phase high-performance liquid chromatographic (rp-HPLC) assay for the determination of baicalin in rat thalamus was developed. This was carried out on a Hypersil -C-18, column using 4-nitro-benzoic acid as the internal standard with a mobile phase of methanol-water-H3PO4 (45:55:0.2, v/v/v). Detection was by UV at 277 nm. The calibration curve for baicalin was linear (r = 0.9992) over the concentration range of 0.05-4.0 mu g/mL and the limit of detection was 10 ng/mL. The coefficients of variation of intra- and inter-day assays were 2.64, 5.19 and 3.19% and 3.46, 6.21 and 5.58% at concentrations of 0.5, 1.0 and 3.0 mu g/mL, respectively. The recoveries of baicalin from rat thalamus were 85.4 +/- 5.62, 90.7 +/- 2.43 and 89.1 +/- 4.75% at concentrations of 0.5, 1.0 and 3.0 mu g/mL, respectively. The method was applied to determine the time course of baicalin in rat thalamus, following a single dosage of intravenous administration of Scutellariae radix extract at 90 mg/kg of baicalin to male Wistar rats. Copyright (c) 2005 John Wiley & Sons, Ltd.
语种英语 ; 英语
出版者JOHN WILEY & SONS LTD ; CHICHESTER ; THE ATRIUM, SOUTHERN GATE, CHICHESTER PO19 8SQ, W SUSSEX, ENGLAND
内容类型期刊论文
源URL[http://hdl.handle.net/123456789/27045]  
专题清华大学
推荐引用方式
GB/T 7714
Zhang, LJ,Xing, DM,Ding, Y,et al. A chromatographic method for baicalin quantification in rat thalamus[J],2010, 2010.
APA Zhang, LJ,Xing, DM,Ding, Y,Wang, RF,Wang, XL,&Du, LJ.(2010).A chromatographic method for baicalin quantification in rat thalamus..
MLA Zhang, LJ,et al."A chromatographic method for baicalin quantification in rat thalamus".(2010).
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