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	The epitope recognized by a monoclonal antibody in influenza A virus M2 protein is immunogenic and confers immune protection
	Zou, P ; Liu, WL ; Chen, YH
	2010-05-11 ; 2010-05-11
关键词	epitope mapping M2e protein influenza virus lethal challenge VACCINE MICE Immunology Pharmacology & Pharmacy
中文摘要	Based on our previous study that the monoclonal antibody (mAb) 8C6 recognizing the extracellular domain of influenza A virus M2 protein (M2e) could passively induce protective immunity in mice, epitope mapping was performed in this study. A series of eight mutated M2e were constructed and expressed. It was found in immunoblotting assay that 8C6 could not bind to two of the mutated M2e proteins, which suggested the VETPIR epitope (aa7-12) in M2e for mAb 8C6. More important, EVETPIRN-peptide (aa6-13) conjugated immunogen induced high M2e specific antibody titer (1:25,600) in mice after booster immunization, and provided mice significantly 40% higher survival rate compared with the control group in challenge assay (p = 0.0215), which suggested the EVETPIRN-epitope was immunogenic and actively conferred immune protection to some extent. In addition, sequence comparison suggested the EVETPIRN sequence (aa6-13) was highly conserved in all human influenza A strains. All these data suggested the EVETPIRN sequence in M2e could be one new target for influenza vaccine design. (C) 2005 Published by Elsevier B.V.
语种	英语 ; 英语
出版者	ELSEVIER SCIENCE BV ; AMSTERDAM ; PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/27017]
专题	清华大学
推荐引用方式 GB/T 7714	Zou, P,Liu, WL,Chen, YH. The epitope recognized by a monoclonal antibody in influenza A virus M2 protein is immunogenic and confers immune protection[J],2010, 2010.
APA	Zou, P,Liu, WL,&Chen, YH.(2010).The epitope recognized by a monoclonal antibody in influenza A virus M2 protein is immunogenic and confers immune protection..
MLA	Zou, P,et al."The epitope recognized by a monoclonal antibody in influenza A virus M2 protein is immunogenic and confers immune protection".(2010).

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