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	The complete pathway for ERK2-catalyzed reaction - Evidence for an iso random BiBi mechanism
	Wang, Zhi-Xin ; Wu, Jia-Wei
	2010-05-11 ; 2010-05-11
关键词	DEPENDENT PROTEIN-KINASE TRANSCRIPTION FACTOR KINETIC MECHANISM CATALYTIC SUBUNIT STEADY-STATE MAP KINASES SUBSTRATE PHOSPHORYLATION EQUILIBRIUM ERK2 Biochemistry & Molecular Biology
中文摘要	In the present study, the enzymatic mechanism of ERK2 is re-examined by a combination of steady-state kinetic studies in the absence and presence of viscosogenic agents. Kinetic studies carried out in various concentrations of sucrose revealed that both k(cat) and k(cat)/K-m for either ATP or Ets Delta 138 were highly sensitive to solvent viscosity, suggesting that the rapid equilibrium assumption is not valid for the phosphorylation of protein substrate by ERK2. Furthermore, the kinetic analysis with the minimal random Bi Bi reaction mechanism is shown to be inconsistent with the principle of the detailed balance. This inconsistent calculation strongly suggests that there is isomerization of the enzyme-substrate ternary complex. The viscosity-dependent steady-state kinetic data are combined to establish a kinetic mechanism for the ERK2-catalyzed reaction that predicts initial reaction velocities under varying concentrations of ATP and substrate. These results complement previous structure-function studies of mitogen-activated protein kinases and provide important insight for mechanistic interpretation of the kinase functions.
语种	英语 ; 英语
出版者	AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC ; BETHESDA ; 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/26980]
专题	清华大学
推荐引用方式 GB/T 7714	Wang, Zhi-Xin,Wu, Jia-Wei. The complete pathway for ERK2-catalyzed reaction - Evidence for an iso random BiBi mechanism[J],2010, 2010.
APA	Wang, Zhi-Xin,&Wu, Jia-Wei.(2010).The complete pathway for ERK2-catalyzed reaction - Evidence for an iso random BiBi mechanism..
MLA	Wang, Zhi-Xin,et al."The complete pathway for ERK2-catalyzed reaction - Evidence for an iso random BiBi mechanism".(2010).

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