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	Single-molecule force spectroscopy study of interaction between transforming growth factor beta 1 and its receptor in living cells
	Yu, Junping ; Wang, Qiang ; Shi, Xiaoli ; Ma, Xinyong ; Yang, Huayan ; Chen, Ye-Guang ; Fang, Xiaohong
	2010-05-11 ; 2010-05-11
关键词	ATOMIC-FORCE RECOGNITION EVENTS DYNAMIC STRENGTH MICROSCOPY ADHESION COMPLEX BINDING DNA TRANSCRIPTION LOCALIZATION Chemistry, Physical
中文摘要	Transforming growth factor beta 1 (TGF-beta 1) regulates many important cellular processes such as cell proliferation, differentiation, and apoptosis, etc. Its signaling is initiated by binding to and bringing together TGF-beta type II receptor (T beta RII) and type I receptor (T beta RI). However, it is not fully understood how the TGF-beta 1 ligand-receptor interaction occurs in living cells and what is the molecular mechanism of the signaling complex TGF-beta 1/T beta RII/T beta RI formation. In this study, we have investigated the interaction between TGF-beta 1 and its receptors in living cells with single-molecule force spectroscopy for the first time. By positioning TGF-beta 1-modified atomic force microscope (AFM) tips on the cells expressing fluorescent protein tagged TGF-beta receptors, the living-cell force measurement was realized with a combined fluorescence microscope and AFM. We found that coexpression of T beta RI with T beta RII enhanced the binding force of TGF-beta 1 with its receptors, hereas the expressed T beta RI itself exhibited no binding affinity to TGF-beta 1. Moreover, the unbinding dynamics of TGF-beta 1/T beta RII and TGF-beta 1/T beta RI/T beta RII were investigated with dynamic force spectroscopy under different AFM loading rates. The dissociation rate constants of TGF-beta 1 with its receptors as well as other parameters characterizing their dissociation pathways were obtained. The results suggested a more stable binding of TGF-beta 1 with the receptor after T beta RI is recruited and the important contribution of T beta RI to the signaling complex formation during TGF-beta I signaling.
语种	英语 ; 英语
出版者	AMER CHEMICAL SOC ; WASHINGTON ; 1155 16TH ST, NW, WASHINGTON, DC 20036 USA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/26975]
专题	清华大学
推荐引用方式 GB/T 7714	Yu, Junping,Wang, Qiang,Shi, Xiaoli,et al. Single-molecule force spectroscopy study of interaction between transforming growth factor beta 1 and its receptor in living cells[J],2010, 2010.
APA	Yu, Junping.,Wang, Qiang.,Shi, Xiaoli.,Ma, Xinyong.,Yang, Huayan.,...&Fang, Xiaohong.(2010).Single-molecule force spectroscopy study of interaction between transforming growth factor beta 1 and its receptor in living cells..
MLA	Yu, Junping,et al."Single-molecule force spectroscopy study of interaction between transforming growth factor beta 1 and its receptor in living cells".(2010).

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