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	Identification and biochemical properties of dps (starvation-induced DNA binding protein) from cyanobacterium Anabaena sp PCC 7120
	Wei, Xiong ; He, Mingjia ; Li, Xiufeng ; Yang, Gao ; Wu, Qingyu
	2010-05-11 ; 2010-05-11
关键词	Anabaena sp PCC 7120 DNA protection Dps ferroxidase iron detoxification ESCHERICHIA-COLI DPS SP STRAIN PCC-7120 OXIDATIVE STRESS IRON INCORPORATION STATIONARY-PHASE LISTERIA-INNOCUA FERRITIN PROTECTION DETOXIFICATION HOMOLOG Biochemistry & Molecular Biology Cell Biology
中文摘要	DNA-binding Proteins from Starved cells (Dps) are anti-stress iron proteins preserving bacteria from oxidative damage. Based on sequence alignment, a 564-bp open reading frame ( all1173) encoding product in Anabaena sp. PCC 7120 shared high similarity with Dps family proteins. RT-PCR showed all1173 is active at transcriptional level in Anabaena sp. PCC 7120 cells. We accordingly cloned the all1173 into prokaryotic expression system, purified the corresponding recombinant protein ( Dps1173) and characterized its properties in vitro. According to CD spectrum and non-denaturing electrophoresis assays, recombinant Dps1173 was alpha helix riched, and was likely to form dodecametric oligomer under native conditions. Fluorescence titration experiment revealed two major iron binding sites within Dps1173 monomer, indicating its potential ferroxidase activity. Although phenomena of direct DNA binding was not observed in Electrophoretic mobility shift assay, Dps1173 could also protect DNA from H2O2 stress for its iron scavenging capacity. This is the first description of Dps from heterocystous cyanobacterium Anabaena sp. PCC 7120.
语种	英语 ; 英语
出版者	TAYLOR & FRANCIS INC ; PHILADELPHIA ; 325 CHESTNUT ST, SUITE 800, PHILADELPHIA, PA 19106 USA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/26940]
专题	清华大学
推荐引用方式 GB/T 7714	Wei, Xiong,He, Mingjia,Li, Xiufeng,et al. Identification and biochemical properties of dps (starvation-induced DNA binding protein) from cyanobacterium Anabaena sp PCC 7120[J],2010, 2010.
APA	Wei, Xiong,He, Mingjia,Li, Xiufeng,Yang, Gao,&Wu, Qingyu.(2010).Identification and biochemical properties of dps (starvation-induced DNA binding protein) from cyanobacterium Anabaena sp PCC 7120..
MLA	Wei, Xiong,et al."Identification and biochemical properties of dps (starvation-induced DNA binding protein) from cyanobacterium Anabaena sp PCC 7120".(2010).

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