| The effect of phosphoryl oxygen on the intermolecular action of alanine and lysozyme | |
| Fang, MJ ; Luo, SN ; Wang, HQ ; Liu, WY ; Zhao, YF | |
| 2010-05-11 ; 2010-05-11 | |
| 关键词 | ESI-MS intermolecular hydrogen bond molecular dynamic simulation IONIZATION MASS-SPECTROMETRY ELECTROSPRAY-IONIZATION COMPLEXES PEPTIDES PROTEINS ACIDS Chemistry, Physical |
| 中文摘要 | It was found that N-diisopropyloxyphosphoryl alanine (DIPP-Ala) could form adduct with hen egg white lysozyme (HEWL) as shown in electrospray ionization mass spectroscopy (ESI-MS), but the non-phosphorylated alanine couldn't. The capability for formation of DIPP-Ala dimmer was more stronger than that of alanine. It suggested that a specific non-covalent complex was formed in the solution phase and could be transferred to the gas phase via electrospray ionization (ESI). The results implied that phosphorylated alanine possessed relatively stronger affinities for protein and formed non-covalent complexes with protein more easily than alanine. Using Tripos force field, molecular mechanics calculation on DIPP-Ala dimmer showed that such non-covalent adduct formation was due to the intermolecular hydrogen bond. |
| 语种 | 英语 ; 英语 |
| 出版者 | PEKING UNIV PRESS ; BEIJING ; PEKING UNIV, CHEMISTRY BUILDING, BEIJING 100871, PEOPLES R CHINA |
| 内容类型 | 期刊论文 |
| 源URL | [http://hdl.handle.net/123456789/26925]  |
| 专题 | 清华大学 |
| 推荐引用方式 GB/T 7714 | Fang, MJ,Luo, SN,Wang, HQ,et al. The effect of phosphoryl oxygen on the intermolecular action of alanine and lysozyme[J],2010, 2010. |
| APA | Fang, MJ,Luo, SN,Wang, HQ,Liu, WY,&Zhao, YF.(2010).The effect of phosphoryl oxygen on the intermolecular action of alanine and lysozyme.. |
| MLA | Fang, MJ,et al."The effect of phosphoryl oxygen on the intermolecular action of alanine and lysozyme".(2010). |
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