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	Oligomerization and aggregation of bovine pancreatic ribonuclease A: Backbone hydration probed by infrared band-shift
	Zhang, Jun ; Yan, Yong-Bin
	2010-05-11 ; 2010-05-11
关键词	protein backbone hydration protein folding protein aggregation infrared spectroscopy infrared amide I' bandshift PROTEIN HYDRATION THERMAL AGGREGATION SEQUENTIAL EVENTS CORRELATION SPECTROSCOPY PREFERENTIAL HYDRATION CONFORMATIONAL-CHANGES SPECTRAL PARAMETERS HELICAL PEPTIDES AQUEOUS-SOLUTION AMYLOID FIBRILS Biochemistry & Molecular Biology
中文摘要	Protein hydration plays a crucial role in almost all aspects of biomolecular processes. In this research, we studied the hydration/dehydration-induced infrared amide I band-shift by using poly-L-lysine and bovine pancreas ribonuclease A as model polypeptides. It was found that a 1-4 cm(-1) shift could be clearly distinguished for all regular secondary structures during protein thermal unfolding. This shift was proven to be due to backbone hydration but not from experimental error, temperature effect or possible incomplete hydrogen/deuterium exchange of the samples. Moreover, we also found that protein aggregation was closely associated with the backbone hydration/dehydration status of proteins. In conditions favoring aggregation, a significant shift to a higher wavenumber of the band from the intermolecular beta-sheet structures in aggregates was observed. The present study suggested that the changes of the amounts of regular secondary structures could be monitored by the intensity changes, while the changes of the hydration status could be monitored by the shift of the infrared bands.
语种	英语 ; 英语
出版者	BENTHAM SCIENCE PUBL LTD ; SHARJAH ; EXECUTIVE STE Y26, PO BOX 7917, SAIF ZONE, 1200 BR SHARJAH, U ARAB EMIRATES
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/26920]
专题	清华大学
推荐引用方式 GB/T 7714	Zhang, Jun,Yan, Yong-Bin. Oligomerization and aggregation of bovine pancreatic ribonuclease A: Backbone hydration probed by infrared band-shift[J],2010, 2010.
APA	Zhang, Jun,&Yan, Yong-Bin.(2010).Oligomerization and aggregation of bovine pancreatic ribonuclease A: Backbone hydration probed by infrared band-shift..
MLA	Zhang, Jun,et al."Oligomerization and aggregation of bovine pancreatic ribonuclease A: Backbone hydration probed by infrared band-shift".(2010).

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