QQ客服 官方微博 反馈留言

	Biosynthesis of polyhydroxyalkanoate copolyesters by Aeromonas hydrophila mutant expressing a low-substrate-specificity PHA synthase PhaC2(Ps)
	Qin, Ling-Fang ; Gao, Xue ; Liu, Qian ; Wu, Qiong ; Chen, Guo-Qiang
	2010-05-11 ; 2010-05-11
关键词	polyhydroxyalkanoates (PHA) Aeronzonas hydrophila fermentation metabolic engineering PHA synthase POLY-BETA-HYDROXYBUTYRATE CHAIN-LENGTH BIOTECHNOLOGICAL PRODUCTION PSEUDOMONAS SP-61-3 ENHANCED PRODUCTION RALSTONIA-EUTROPHA ESCHERICHIA-COLI POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYHEXANOATE) ACID PSEUDOMONAS-STUTZERI-1317 Biotechnology & Applied Microbiology Engineering, Chemical
中文摘要	A polyhydroxyalkanoate (PHA) synthase negative mutant termed Aeromonas hydrophila CQ4 was constructed from its wild type strain A. hydrophila 4AK4. Heterologous expression of a low-substrate-specificity PHA synthase PhaC2(Ps), cloned from Pseudomonas stutzeri 1317 in A. hydrophila CQ4 could copolymerize short-chain-length (SCL) 3-hydroxybutyrate (3HB) and medium-chain-length (MCL) 3-hydroxyalkanoates (3HA). Co-expressing (R)-specific enoyl-CoA hydratase and PHA synthase PhaC2(Ps) in A. hydrophila CQ4 (phaJ(Ah), phaC2(Ps)) led to accumulation of 20.86 wt% PHA copolyesters containing 59 mol% 3HB, 37 mo1% 3-hydroxyhexanoate (3HHx) and 4 mol% of other MCL 3HA in shake flask culture. When grown in fermentor, cellular dry weight (CDW) and PHA content were 22.75 g L-1 and 20.08 wt%, respectively, with 72.38 mol% 3HB, 25.18 mol% 3HHx and 2.45 mol% other MCL 3HA in the copolymer. If beta-ketothiolase and acetoacetyl-CoA reductase of Ralstonia eutropha were co-expressed with PhaC2(Ps), A. hydrophila CQ4 (phaC2(Ps), phbA(Re), phbB(Re)) accumulated PHA copolyesters containing enhanced 3HB content up to 79.95 mol% and reduced 3HHx fraction of 8.55 mol%. When grown in fermentor it produced 16.28 g L-1 CDW containing 11.64 wt% PHA consisting of 87.38 mol% 3HB, 9.75 mol% 3HHx and 2.87 mol% other MCL 3HA. These results further demonstrate that A. hydrophila is a good candidate for metabolic engineering for diverse PHA production. (c) 2007 Elsevier B.V. All rights reserved.
语种	英语 ; 英语
出版者	ELSEVIER SCIENCE SA ; LAUSANNE ; PO BOX 564, 1001 LAUSANNE, SWITZERLAND
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/26918]
专题	清华大学
推荐引用方式 GB/T 7714	Qin, Ling-Fang,Gao, Xue,Liu, Qian,et al. Biosynthesis of polyhydroxyalkanoate copolyesters by Aeromonas hydrophila mutant expressing a low-substrate-specificity PHA synthase PhaC2(Ps)[J],2010, 2010.
APA	Qin, Ling-Fang,Gao, Xue,Liu, Qian,Wu, Qiong,&Chen, Guo-Qiang.(2010).Biosynthesis of polyhydroxyalkanoate copolyesters by Aeromonas hydrophila mutant expressing a low-substrate-specificity PHA synthase PhaC2(Ps)..
MLA	Qin, Ling-Fang,et al."Biosynthesis of polyhydroxyalkanoate copolyesters by Aeromonas hydrophila mutant expressing a low-substrate-specificity PHA synthase PhaC2(Ps)".(2010).

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。