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	Parallel profiling of active transcription factors using an oligonucleotide array-based transcription factor assay (OATFA)
	Shao, W ; Wei, HJ ; Qiao, JY ; Zhao, YC ; Sun, YM ; Zhou, YX ; Cheng, J
	2010-05-11 ; 2010-05-11
关键词	transcription factor EMSA DNA-binding activity oligonucleotide array oligonucleotide array-based transcription factor assay (OATFA) MOBILITY SHIFT ASSAY GEL-ELECTROPHORESIS GENE-EXPRESSION C-JUN BINDING GENOME PHOSPHORYLATION PROTEINS Biochemical Research Methods
中文摘要	Cell/tissue-specific gene expression are tightly regulated by various combinations of multiple transcription factors (TFs). Here, we present an oligonucleotide array-based transcription factor assay (OATFA), which allows the simultaneous assay of multiple active TFs. In this proof-of-principle work, both purified TFs and cell extracts were analyzed using OATFA and further antibody-based validation confirmed the chip data. This method could simplify the assay of multiple TFs and may facilitate high-throughput profiling of large numbers of TFs.
语种	英语 ; 英语
出版者	AMER CHEMICAL SOC ; WASHINGTON ; 1155 16TH ST, NW, WASHINGTON, DC 20036 USA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/26914]
专题	清华大学
推荐引用方式 GB/T 7714	Shao, W,Wei, HJ,Qiao, JY,et al. Parallel profiling of active transcription factors using an oligonucleotide array-based transcription factor assay (OATFA)[J],2010, 2010.
APA	Shao, W.,Wei, HJ.,Qiao, JY.,Zhao, YC.,Sun, YM.,...&Cheng, J.(2010).Parallel profiling of active transcription factors using an oligonucleotide array-based transcription factor assay (OATFA)..
MLA	Shao, W,et al."Parallel profiling of active transcription factors using an oligonucleotide array-based transcription factor assay (OATFA)".(2010).

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