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	Leucine zipper domain is important for actin binding of p57
	Liu, CZ ; He, L ; Shen, QT ; Li, XH ; Sui, SF
	2010-05-11 ; 2010-05-11
关键词	p57 actin binding activity leucine zipper domain CORONIN FAMILY TRANSIENT ASSOCIATION WD REPEAT PROTEIN PHAGOSOMES MOTIF DIMERIZATION SUBUNITS SURVIVAL COMMON Biochemistry & Molecular Biology Biophysics
中文摘要	The role of the leucine zipper domain in actin binding of p57 was investigated by the methods of the F-actin co-sedimentation studies in vitro, the immunofluorescence co-localization analyses in cells, the Western blot and density scan assays. The results showed that the leucine zipper-containing domain alone has no actin-binding activity, while the C-terminal deletion mutation removing this domain or the point mutation destroying leucine zipper domain leads to great reduction of actin-binding activity of p57. Furthermore, the degrees of degradation in actin-binding activity caused by the two mutations were assessed in quasi-quantitative analysis in vitro and in cells and both mutations exhibited the extremely similar depressing effects. These data strongly suggest that the leucine zipper domain is important for actin binding of p57.
语种	中文 ; 中文
出版者	SCIENCE PRESS ; BEIJING ; 16 DONGHUANGCHENGGEN NORTH ST, BEIJING 100717, PEOPLES R CHINA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/26901]
专题	清华大学
推荐引用方式 GB/T 7714	Liu, CZ,He, L,Shen, QT,et al. Leucine zipper domain is important for actin binding of p57[J],2010, 2010.
APA	Liu, CZ,He, L,Shen, QT,Li, XH,&Sui, SF.(2010).Leucine zipper domain is important for actin binding of p57..
MLA	Liu, CZ,et al."Leucine zipper domain is important for actin binding of p57".(2010).

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