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	smad2 and smad3 are required for mesendoderm induction by transforming growth factor-beta/Nodal signals in zebrafish
	Jia, Shunji ; Ren, Zhen ; Li, Xiang ; Zheng, Ying ; Meng, Anming
	2010-05-11 ; 2010-05-11
关键词	ONE-EYED-PINHEAD NODAL-RELATED SIGNALS TGF-BETA VERTEBRATE DEVELOPMENT TARGETED DISRUPTION MESODERM INDUCTION GENE-EXPRESSION AXIS FORMATION MOUSE EMBRYO IDENTIFICATION Biochemistry & Molecular Biology
中文摘要	The transforming growth factor-beta ligands Nodal, activin, and Vg1 play important roles in mesendoderm induction and patterning during vertebrate embryogenesis. These ligands are believed to transduce the signal through the receptor-activated transcription factors Smad2 and Smad3. However, the roles of smad2/ 3 genes in development of zebrafish embryos are largely unknown because the presence of multiple smad2/ 3 genes and their maternal expression have hampered the investigation of their developmental roles. We generated potent and specific dominant- negative forms of zebrafish Smad2, Smad3a, and Smad3b by mutating multiple amino acids. Overexpression of these mutants abolished mesendoderm induction by ectopic Nodal signaling in zebrafish embryos. Expression of dominantnegative smad2/3 abrogated Smad2/3 activities in wild- type embryos and caused various mesendodermal defects similar to those in Nodal- deficient embryos. Smad2/3-deficient cells transplanted into the blastodermal margin of wild- type hosts preferentially differentiated into ectodermal tissues rather than mesendodermal tissues, supporting the idea that response of cells to mesendoderm inducers requires Smad2/3 activities. Interference with Smad2/3 activities in Zoep, Moep, and MZoep mutant embryos resulted in more severe mesendodermal defects. Thus, our data reveal that Nodal signaling and mesendoderm induction depend on Smad2/3 and suggest that transforming growth factor-beta signals other than Nodal also contribute to Smad2/3 signaling and embryonic patterning.
语种	英语 ; 英语
出版者	AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC ; BETHESDA ; 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/26887]
专题	清华大学
推荐引用方式 GB/T 7714	Jia, Shunji,Ren, Zhen,Li, Xiang,et al. smad2 and smad3 are required for mesendoderm induction by transforming growth factor-beta/Nodal signals in zebrafish[J],2010, 2010.
APA	Jia, Shunji,Ren, Zhen,Li, Xiang,Zheng, Ying,&Meng, Anming.(2010).smad2 and smad3 are required for mesendoderm induction by transforming growth factor-beta/Nodal signals in zebrafish..
MLA	Jia, Shunji,et al."smad2 and smad3 are required for mesendoderm induction by transforming growth factor-beta/Nodal signals in zebrafish".(2010).

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