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	An ankyrin repeat-containing protein, characterized as a ubiquitin ligase, is closely associated with membrane-enclosed organelles and required for pollen germination and pollen tube growth in lily
	Huang, J ; Chen, F ; Del Casino, C ; Autino, A ; Shen, M ; Yuan, S ; Peng, J ; Shi, H ; Wang, C ; Cresti, M ; Li, Y
	2010-05-11 ; 2010-05-11
关键词	ACTIN-DEPOLYMERIZING FACTOR GENE-EXPRESSION LILIUM-LONGIFLORUM UBIQUITIN/PROTEASOME PATHWAY PECTIN METHYLESTERASE NICOTIANA-TABACUM PLANT-CELLS CAP-TRAPPER TIP GROWTH ARABIDOPSIS Plant Sciences
中文摘要	Exhibiting rapid polarized growth, the pollen tube delivers the male gametes into the ovule for fertilization in higher plants. To get an overall picture of gene expression during pollen germination and pollen tube growth, we profiled the transcription patterns of 1,536 pollen cDNAs from lily (Lilium longiflorum) by microarray. Among those that exhibited significant differential expression, a cDNA named lily ankyrin repeat-containing protein (LlANK) was thoroughly studied. The full-length LlANK cDNA sequence predicts a protein containing five tandem ankyrin repeats and a RING zinc-finger domain. The LlANK protein possesses ubiquitin ligase activity in vitro. RNA blots demonstrated that LlANK transcript is present in mature pollen and its level, interestingly contrary to most pollen mRNAs, up-regulated significantly during pollen germination and pollen tube growth. When fused with green fluorescent protein and transiently expressed in pollen, LlANK was found dominantly associated with membrane-enclosed organelles as well as the generative cell. Overexpression of LlANK, however, led to abnormal growth of the pollen tube. On the other hand, transient silencing of LlANK impaired pollen germination and tube growth. Taken together, these results showed that LlANK is a ubiquitin ligase associated with membrane-enclosed organelles and required for polarized pollen tube growth.
语种	英语 ; 英语
出版者	AMER SOC PLANT BIOLOGISTS ; ROCKVILLE ; 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/26880]
专题	清华大学
推荐引用方式 GB/T 7714	Huang, J,Chen, F,Del Casino, C,et al. An ankyrin repeat-containing protein, characterized as a ubiquitin ligase, is closely associated with membrane-enclosed organelles and required for pollen germination and pollen tube growth in lily[J],2010, 2010.
APA	Huang, J.,Chen, F.,Del Casino, C.,Autino, A.,Shen, M.,...&Li, Y.(2010).An ankyrin repeat-containing protein, characterized as a ubiquitin ligase, is closely associated with membrane-enclosed organelles and required for pollen germination and pollen tube growth in lily..
MLA	Huang, J,et al."An ankyrin repeat-containing protein, characterized as a ubiquitin ligase, is closely associated with membrane-enclosed organelles and required for pollen germination and pollen tube growth in lily".(2010).

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