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	A novel matrix protein p10 from the nacre of pearl oyster (Pinctada fucata) and its effects on both CaCO3 crystal formation and mineralogenic cells
	Zhang, Cen ; Li, Shuo ; Ma, Zhuojun ; Xie, Liping ; Zhang, Rongqing
	2010-05-11 ; 2010-05-11
关键词	aragonite biomineralization bone formation nacre soluble matrix proteins WATER-SOLUBLE MATRIX ALKALINE-PHOSPHATASE ACTIVITY OSTEOBLASTS MAINTAINED INVITRO CALCIUM-CARBONATE CRYSTALS ORGANIC MATRIX MOLLUSK-SHELL HALIOTIS-TUBERCULATA LAYER FORMATION MC3T3-E1 CELLS MAXIMA Biotechnology & Applied Microbiology Marine & Freshwater Biology
中文摘要	A novel matrix protein, designated as p 10 because of its apparent molecular mass of 10 kDa, was isolated from the nacreous layer of pearl oyster (Pinctada fucata) by reverse-phase high-performance liquid chromatography. In vitro crystallization experiments showed that p10 could accelerate the nucleation of calcium carbonate crystals and induce aragonite formation, suggesting that it might play a key role in nacre biomineralization. As nacre is known to contain osteogenic factors, two mineralogenic cell lines, MRC-5 fibroblasts and MCM-E1 preosteoblasts, were used to investigate the biological activity of p10. The results showed that p10 could increase alkaline phosphatase activity, an early marker of osteoblast differentiation, while the viability of MRC-5 and MC3T3-E1 remained unchanged after treatment of p10. Taken together, the findings led to identification of a novel matrix protein from the nacre of P. fucata that plays a role in both the mineral phase and in the differentiation of the cells involved in biomineralization.
语种	英语 ; 英语
出版者	SPRINGER ; NEW YORK ; 233 SPRING STREET, NEW YORK, NY 10013 USA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/26832]
专题	清华大学
推荐引用方式 GB/T 7714	Zhang, Cen,Li, Shuo,Ma, Zhuojun,et al. A novel matrix protein p10 from the nacre of pearl oyster (Pinctada fucata) and its effects on both CaCO3 crystal formation and mineralogenic cells[J],2010, 2010.
APA	Zhang, Cen,Li, Shuo,Ma, Zhuojun,Xie, Liping,&Zhang, Rongqing.(2010).A novel matrix protein p10 from the nacre of pearl oyster (Pinctada fucata) and its effects on both CaCO3 crystal formation and mineralogenic cells..
MLA	Zhang, Cen,et al."A novel matrix protein p10 from the nacre of pearl oyster (Pinctada fucata) and its effects on both CaCO3 crystal formation and mineralogenic cells".(2010).

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