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	A study of specific interaction of the transcription factor and the DNA element by atomic force microscopy
	Qin Feng ; Jiang Yaxin ; Ma Xinyong ; Chen Feng ; Fang Xiaohong ; Bai Chunli ; Li Yiqin
	2010-05-11 ; 2010-05-11
关键词	Experimental Theoretical or Mathematical/ atomic force microscopy DNA electrophoresis molecular biophysics statistical analysis/ atomic force microscopy transcription factor ZmDREBlA DNA sequence binding probability core sequence ACCGAC ERE element AGCCGCC single-molecular unbinding force Poisson statistical method electrophoretic mobility shift functional analysis/ A8715B Biomolecular structure, configuration, conformation, and active sites A8715K Biomolecular interactions, charge transfer complexes A3620E Macromolecular constitution (chains and sequences) A3620H Macromolecular configuration (bonds, dimensions) A0250 Probability theory, stochastic processes, and statistics A8245 Electrochemistry and electrophoresis
中文摘要	Atomic force microscopy (AFM) was employed to quantitatively detect the interaction between a transcription factor, ZmDREBlA, and its target DNA sequence (DRE). The specificity of the interaction was revealed by comparing the binding probability of ZmDREBlA to DRE element with core sequence ACCGAC and to the ERE element with core sequence AGCCGCC. Moreover, the single-molecular unbinding force between ZmDREBlA and DRE element was determined using Poisson statistical method to be 99+/-9 pN, The results indicated that AFM could serve as a sensitive and reliable technique to probe the interaction properties of the transcription factor and DNA element at single-molecular level, thus indicating that AFM is advantageous over the traditional methods, such as the electrophoretic mobility shift assay, regarding to the functional analysis of transcription factors.
语种	英语 ; 英语
出版者	Science Press ; China
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/26814]
专题	清华大学
推荐引用方式 GB/T 7714	Qin Feng,Jiang Yaxin,Ma Xinyong,et al. A study of specific interaction of the transcription factor and the DNA element by atomic force microscopy[J],2010, 2010.
APA	Qin Feng.,Jiang Yaxin.,Ma Xinyong.,Chen Feng.,Fang Xiaohong.,...&Li Yiqin.(2010).A study of specific interaction of the transcription factor and the DNA element by atomic force microscopy..
MLA	Qin Feng,et al."A study of specific interaction of the transcription factor and the DNA element by atomic force microscopy".(2010).

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