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	Analysis of the cotton E6 promoter
	Wu Aimin ; Liu Jinyuan
	2010-05-11 ; 2010-05-11
关键词	Practical/ biotechnology botany cellular biophysics cotton fluorescence genetic engineering proteins quality control/ cotton E6 promoter E6 gene sea island cotton Gossypium barbadense cotton fiber cells species cultivation transgenic engineering 614-bp fragment flanking region upland cotton Gossypium hirsutum CRI-12 green fluorescent protein transgenic tobacco seedlings fluorescent analysis leaf trichomes hair cells transgenic tobacco plants unicellular trichomes epidermal cells cotton ovules genetic engineering cotton fiber quality/ E3628 Biotechnology industry E3010 Agriculture
中文摘要	An E6 gene from sea island cotton (Gossypium barbadense) was expressed specifically in cotton fiber cells to transfer functions to cultivated species for better transgenic engineering. The regulatory activity of the E6 promoter region was then studied by isolating a 614-bp fragment of the 5'-flanking region from upland cotton (Gossypium hirsutum CRI-12) to produce a green fluorescent protein (GFP) reporter construct for analysis of tissue-specific expression in transgenic tobacco seedlings. Fluorescent analyses indicate that the relatively short E6 promoter is sufficient to direct green fluorescent protein expression specifically in the leaf trichomes (hair cells) of the transgenic tobacco plants. As cotton fibers are also unicellular trichomes that differentiate from epidermal cells of developing cotton ovules, the result suggests that the relatively short E6 promoter can serve as a fiber-specific expression promoter for genetic engineering to improve cotton fiber quality.
语种	英语 ; 英语
出版者	Editorial Board of J. of Tsinghua University ; China
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/26793]
专题	清华大学
推荐引用方式 GB/T 7714	Wu Aimin,Liu Jinyuan. Analysis of the cotton E6 promoter[J],2010, 2010.
APA	Wu Aimin,&Liu Jinyuan.(2010).Analysis of the cotton E6 promoter..
MLA	Wu Aimin,et al."Analysis of the cotton E6 promoter".(2010).

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