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	Genomic screen for expression pattern in adult Drosophila brains in vivo by enhancer trap method and establishment of expression database
	Liu Xiao-Jun ; Yuan Xiao-Jing ; Sun Kan ; Shuai Yi-Chun ; Song Qing-Xuan ; Wang Lei ; Shao Li-Sha ; Zhao Xin-Yu ; Lue Yi-Sheng ; Lue Yu-Bing ; Wang Lian-Zhang ; Zhong Yi
	2010-05-11 ; 2010-05-11
关键词	enhancer trap method mutant libraries reporter transgenic flies mushroom body expression pattern MUSHROOM BODIES MEMORY NEUROTRANSMISSION MELANOGASTER Biochemistry & Molecular Biology Biophysics
中文摘要	Enhancer trap is an important technique to build and explore Drosophila brain gene expression pattern and its database. Large genome-wide screen for specific expression pattern, in adult Drosophila brains by enhancer trap method provides fly tools for further understanding neural basis of learning and memory. Enhancer trap method was utilized to generate Drosophila P[Gal4] mutant library. Each mutant in library was crossed with GFP reporter flies (UAS-eGFP); heads of the adult off-springs were dissected to obtain the whole brain which were examined under the epifluorescence microscope and expression pattern of each mutant were further analyzed. JavaScript was utilized to establish an expression pattern library. A total of 2 677 mutant lines of Drosophila were obtained by P-element induced mutagenesis and subjected to expression pattern analysis. Most of mutated genes express in adult fly. 368 mutated genes specific express in the mushroom body, which was reported to involve in Drosophila olfactory learning and memory, and some others specific express in other neuron circuitries. Drosophila mutant libraries and genome-wide expression pattern database was built by enhancer trap method, which as a tool will be utilized to understand the function of genes and particular neural circuitries in Drosophila in the future.
语种	中文 ; 中文
出版者	CHINESE ACAD SCIENCES, INST BIOPHYSICS ; BEIJING ; 15 DATUN RD, CHAOYAND DISTRICT, BEIJING, 100101, PEOPLES R CHINA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/26769]
专题	清华大学
推荐引用方式 GB/T 7714	Liu Xiao-Jun,Yuan Xiao-Jing,Sun Kan,et al. Genomic screen for expression pattern in adult Drosophila brains in vivo by enhancer trap method and establishment of expression database[J],2010, 2010.
APA	Liu Xiao-Jun.,Yuan Xiao-Jing.,Sun Kan.,Shuai Yi-Chun.,Song Qing-Xuan.,...&Zhong Yi.(2010).Genomic screen for expression pattern in adult Drosophila brains in vivo by enhancer trap method and establishment of expression database..
MLA	Liu Xiao-Jun,et al."Genomic screen for expression pattern in adult Drosophila brains in vivo by enhancer trap method and establishment of expression database".(2010).

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