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	Identification of the HIV-1 gp41 core-binding motif-HXXNPF
	Huang, Jing-He ; Liu, Zu-Qiang ; Liu, Shuwen ; Jiang, Shibo ; Chen, Ying-Hua
	2010-05-11 ; 2010-05-11
关键词	HIV-1 gp41 core structure motif phage display IMMUNODEFICIENCY-VIRUS TYPE-1 ENVELOPE GLYCOPROTEIN MEMBRANE-FUSION TRANSMEMBRANE GLYCOPROTEIN MONOCLONAL-ANTIBODY SYNTHETIC PEPTIDE 6-HELIX BUNDLE COILED-COIL ENTRY DOMAIN Biochemistry & Molecular Biology Biophysics Cell Biology
中文摘要	The HIV-1 gp41 core, a six-helix bundle formed between the N- and C-terminal heptad repeats, plays a critical role in fusion between the viral and target cell membranes. Using N36(L8)C34 as a model of the gp41 core to screen phage display peptide libraries, we identified a common motif, HXXNPF (X is any of the 20 natural amino acid residues). A selected positive phage clone L7.8 specifically bound to N36(L8)C34 and this binding could be blocked by a gp41 core-specific monoclonal antibody (NC-1). JCH-4, a peptide containing HXXNPF motif, effectively inhibited HIV-1 envelope glycoprotein-mediated syncytium-formation. The epitope of JCH-4 was proven to be linear and might locate in the NHR regions of the gp41 core. These data suggest that HXXNPF motif may be a gp41 core-binding sequence and HXXNPF motif-containing molecules can be used as probes for studying the role of the HIV-1 gp41 core in membrane-fusion process. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
语种	英语 ; 英语
出版者	ELSEVIER SCIENCE BV ; AMSTERDAM ; PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/26763]
专题	清华大学
推荐引用方式 GB/T 7714	Huang, Jing-He,Liu, Zu-Qiang,Liu, Shuwen,et al. Identification of the HIV-1 gp41 core-binding motif-HXXNPF[J],2010, 2010.
APA	Huang, Jing-He,Liu, Zu-Qiang,Liu, Shuwen,Jiang, Shibo,&Chen, Ying-Hua.(2010).Identification of the HIV-1 gp41 core-binding motif-HXXNPF..
MLA	Huang, Jing-He,et al."Identification of the HIV-1 gp41 core-binding motif-HXXNPF".(2010).

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