| Influence of ultraviolet-C on structure and function of Synechococcus sp PCC 7942 photolyase | |
| Zhang, L. W. ; Li, M. ; Wu, Q. Y. | |
| 2010-05-11 ; 2010-05-11 | |
| 关键词 | circular dichroism (CD) spectrum cyclobutane pyrimidine dimer (CPD) photolyase fluorescence spectrum Synechococcus sp PCC 7942 ultraviolet resistance DNA PHOTOLYASE RADIATION BINDING Biochemistry & Molecular Biology |
| 中文摘要 | In this work an over-expressed cyclobutane pyrimidine dimer (CPD) photolyase of Synechococcus sp. PCC 7942 was used to investigate UV-C (ultraviolet irradiation of C-region) influence on photoreactivation. In vivo photo reactivation experiments indicated that the survival rate decreased from 100 to 2.6% when the UV-C flux was increased from 1.1 to 68.5 mu W/cm(2). It seemed that the photolyase was easily inactivated at UV-C intensities >= 25.5 mu W/cm(2). Spectrometric analysis indicated that tertiary structure of the photolyase changed evidently when the UV-C fluxes were >= 25.5 mu W/cm(2), while the secondary structure was almost unchanged even at 170 mu W/cm(2). Band shift assay indicated that catalytic activity of the photolyase was impaired at fluxes >= 25.5 mu W/cm(2), but no significant influence on DNA-binding activity was observed. These results suggest that photoreactivation is efficient at UV-C fluxes <= 25.5 mu W/cm(2), but would be impaired by intense UV-C irradiation due to structure changes of the photolyase. |
| 语种 | 英语 ; 英语 |
| 出版者 | MAIK NAUKA/INTERPERIODICA/SPRINGER ; NEW YORK ; 233 SPRING ST, NEW YORK, NY 10013-1578 USA |
| 内容类型 | 期刊论文 |
| 源URL | [http://hdl.handle.net/123456789/26720]  |
| 专题 | 清华大学 |
| 推荐引用方式 GB/T 7714 | Zhang, L. W.,Li, M.,Wu, Q. Y.. Influence of ultraviolet-C on structure and function of Synechococcus sp PCC 7942 photolyase[J],2010, 2010. |
| APA | Zhang, L. W.,Li, M.,&Wu, Q. Y..(2010).Influence of ultraviolet-C on structure and function of Synechococcus sp PCC 7942 photolyase.. |
| MLA | Zhang, L. W.,et al."Influence of ultraviolet-C on structure and function of Synechococcus sp PCC 7942 photolyase".(2010). |
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