QQ客服 官方微博 反馈留言

	Microwave-enhanced ink staining for fast and sensitive protein quantification in proteomic studies
	Wu, Xue-Ping ; Cheng, Yong-Sheng ; Liu, Jin-Yuan
	2010-05-11 ; 2010-05-11
关键词	microwave ink staining protein quantification IEF rehydration buffer SDS-PAGE sample buffer quantitative proteomics SODIUM DODECYL-SULFATE NITROCELLULOSE MEMBRANE REDUCING AGENTS DYE-BINDING ASSAY QUANTITATION ACID IDENTIFICATION INTERFERENCE HYDROLYSIS Biochemical Research Methods
中文摘要	A novel microwave-enhanced ink staining method was developed for rapid and sensitive estimation of protein content in sample buffers containing chaotropes, dyes, detergents, and reducing agents. Dye-based Blue-Black ink was used to quantitatively visualize proteins spotted on a nitrocellulose membrane. The total staining time was greatly reduced to 3 min by brief exposure to microwave radiation. The stained membrane was washed with distilled water, baked in a microwave oven for complete desiccation, transparentized with mineral oil, and documented by a desktop scanner or densitometer. Only 1 mu L of protein sample (protein solubilized in SDS-PAGE sample buffer or IEF rehydration buffer) was used for protein spotting. The novel solid-phase protein assay gives a 500-fold dynamic range from 19.5 to 10000 ng/mu L and can be scaled up for high-throughput protein quantification analysis. The fast, sensitive and low-cost microwave-enhanced ink staining procedure is ideal for protein quantification in proteomic analysis.
语种	英语 ; 英语
出版者	AMER CHEMICAL SOC ; WASHINGTON ; 1155 16TH ST, NW, WASHINGTON, DC 20036 USA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/26713]
专题	清华大学
推荐引用方式 GB/T 7714	Wu, Xue-Ping,Cheng, Yong-Sheng,Liu, Jin-Yuan. Microwave-enhanced ink staining for fast and sensitive protein quantification in proteomic studies[J],2010, 2010.
APA	Wu, Xue-Ping,Cheng, Yong-Sheng,&Liu, Jin-Yuan.(2010).Microwave-enhanced ink staining for fast and sensitive protein quantification in proteomic studies..
MLA	Wu, Xue-Ping,et al."Microwave-enhanced ink staining for fast and sensitive protein quantification in proteomic studies".(2010).

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。