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	Candidate peptide-vaccine induced potent protection against CSFV and identified a principal sequential neutralizing determinant on E2
	Dong, XN ; Qi, Y ; Ying, R ; Chen, X ; Chen, YH
	2010-05-11 ; 2010-05-11
关键词	classical swine fever virus (CSFV) marker vaccine peptide vaccine sequential neutralizing epitope immunodominant HOG-CHOLERA VIRUS SWINE-FEVER VIRUS PROTEINS E-RNS ENVELOPE GLYCOPROTEIN IMMUNODEFICIENCY-VIRUS MONOCLONAL-ANTIBODIES IMMUNE-RESPONSE PESTIVIRUS EPITOPE E1 Immunology Medicine, Research & Experimental Veterinary Sciences
中文摘要	Previously, two candidate multi-peptide-vaccines (MPVs) consisted of five overlapping synthetic peptides covering the antigenic domain B/C (aa693-777) on envelope protein E2 were prepared in our lab. And they successfully induced peptide-specific neutralizing antibodies and provided pigs with complete protection from the lethal challenge of virulent classical swine fever virus (CSFV) strain Shimen. In this study, these five peptides were conjugated to bovine serum albumin (BSA), with which five groups of pigs (n = 10) were inoculated, respectively. Among these candidate peptide-vaccines (PVs), PV-BC1 (BC1: aa693-716) exhibited the most potent protective activity, PV-BC3, PV-BC4 and PV-BC5 (BC3: aa723-745; BC4: aa741-760; BC5: aa757-777) had weaker effects, while no effect of PV-BC2 (BC2: aa712-727) had been detected. Moreover, the polyclonal antibodies induced by PV-BC1 and PV-BC4 were capable of neutralizing C-strain virus in vitro. Thus, a principal sequential neutralizing determinant (aa693-716) and a minor sequential neutralizing determinant (aa741-760) were proved to lie in the antigenic domain B/C, which can be recruited into developing more effective "marker vaccine" by epitope-vaccine strategy. Our study also indicates that scanning with a panel of sequential peptide-immunogens is an effective method to locate sequential neutralizing epitopes. (c) 2005 Elsevier Ltd. All rights reserved.
语种	英语 ; 英语
出版者	ELSEVIER SCI LTD ; OXFORD ; THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/26709]
专题	清华大学
推荐引用方式 GB/T 7714	Dong, XN,Qi, Y,Ying, R,et al. Candidate peptide-vaccine induced potent protection against CSFV and identified a principal sequential neutralizing determinant on E2[J],2010, 2010.
APA	Dong, XN,Qi, Y,Ying, R,Chen, X,&Chen, YH.(2010).Candidate peptide-vaccine induced potent protection against CSFV and identified a principal sequential neutralizing determinant on E2..
MLA	Dong, XN,et al."Candidate peptide-vaccine induced potent protection against CSFV and identified a principal sequential neutralizing determinant on E2".(2010).

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