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	Sequential events in ribonuclease A thermal unfolding characterized by two-dimensional infrared correlation spectroscopy
	Zhang, J ; He, HW ; Wang, Q ; Yan, YB
	2010-05-11 ; 2010-05-11
关键词	bovine pancreatic ribonuclease A infrared thermal stability two-dimensional correlation spectroscopy HID exchange HYDROGEN-DEUTERIUM EXCHANGE PROTEIN SECONDARY STRUCTURE BOVINE PANCREATIC RIBONUCLEASE CONFORMATIONAL-CHANGES IR SPECTROSCOPY AGGREGATION STATE HEAT RESOLUTION PHOSPHATE Biochemistry & Molecular Biology
中文摘要	The conformational changes in the thermal denaturation of bovine pancreatic ribonuclease A was followed with infrared spectra and analyzed by second derivative and two-dimensional correlation techniques. By analyzing the sequential events in each transition stage, the results were consistent with a step-wise thermal denaturation mechanism in which the structural adjustment of the N-terminal and the opening of the central structure of the protein come before the main unfolding process. Non-native turns were found to form along with the unfolding of the native structures. The central region that is composed of some beta-sheet and alpha-helical structures was found to be the most stable part that might form the residual structure at high temperatures.
语种	英语 ; 英语
出版者	BENTHAM SCIENCE PUBL LTD ; SHARJAH ; EXECUTIVE STE Y26, PO BOX 7917, SAIF ZONE, 1200 BR SHARJAH, U ARAB EMIRATES
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/26691]
专题	清华大学
推荐引用方式 GB/T 7714	Zhang, J,He, HW,Wang, Q,et al. Sequential events in ribonuclease A thermal unfolding characterized by two-dimensional infrared correlation spectroscopy[J],2010, 2010.
APA	Zhang, J,He, HW,Wang, Q,&Yan, YB.(2010).Sequential events in ribonuclease A thermal unfolding characterized by two-dimensional infrared correlation spectroscopy..
MLA	Zhang, J,et al."Sequential events in ribonuclease A thermal unfolding characterized by two-dimensional infrared correlation spectroscopy".(2010).

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