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	Protective effects of mycosporine-like amino acids of Synechocystis sp PCC 6803 and their partial characterization
	Zhang, Lianwen ; Li, Lianzhi ; Wu, Qingyu
	2010-05-11 ; 2010-05-11
关键词	antioxidation high performance liquid chromatography mass spectra mycosporine-like amino acids Synechocystis sp PCC 6803 UV-photoprotection RADIATION Biochemistry & Molecular Biology Biophysics
中文摘要	In this work, mycosporine-like amino acids (MAAs) of Synechocystis sp. PCC 6803 were characterized and were investigated on UV induction and protective ability. High performance liquid chromatographic (HPLC) studies revealed three major compounds in the MAAs. By UV absorption and mass spectra analysis, one of the compounds was tentatively identified as mycosporine-tau (M-tau). One novel compound similar to usujirene was tentatively named as dehydroxylusujirene, and the other novel compound was named as M-343 according to its absorption maximum. In vivo experiments indicated that M-tau was induced by both UVA and UV-B, while dehydroxylustijirene and M-343 were only induced by UV-A, suggesting that different chromophores were involved in MAAs synthesis in Synechocystis sp. PCC 6803. It was also indicated that M-343 could be photochemically synthesized from some precursors. Under both UV and oxidation stresses, M-343 was more stable than dehydroxylusujirene and M-tau. Considering the reaction with H2O2, Wan and dehydroxylusujirene might be potential antioxidants in reaction with physiological reactive oxygen species in vivo. In protection experiments, the MAAs exhibited efficient protective ability towards UV-B and H2O2 stresses, with maximal protection rates of 30% and 21.5%, respectively. These results indicate that the MAAs in Synechocystis sp. PCC 6803 act as both UV-screen and antioxidant. (c) 2006 Elsevier B.V. All rights reserved.
语种	英语 ; 英语
出版者	ELSEVIER SCIENCE SA ; LAUSANNE ; PO BOX 564, 1001 LAUSANNE, SWITZERLAND
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/26647]
专题	清华大学
推荐引用方式 GB/T 7714	Zhang, Lianwen,Li, Lianzhi,Wu, Qingyu. Protective effects of mycosporine-like amino acids of Synechocystis sp PCC 6803 and their partial characterization[J],2010, 2010.
APA	Zhang, Lianwen,Li, Lianzhi,&Wu, Qingyu.(2010).Protective effects of mycosporine-like amino acids of Synechocystis sp PCC 6803 and their partial characterization..
MLA	Zhang, Lianwen,et al."Protective effects of mycosporine-like amino acids of Synechocystis sp PCC 6803 and their partial characterization".(2010).

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