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	Yeast AMID homologue Ndi1p displays respiration-restricted apoptotic activity and is involved in chronological aging
	Li, W ; Sun, LB ; Liang, QL ; Wang, J ; Mo, WK ; Zhou, B
	2010-05-11 ; 2010-05-11
关键词	PROGRAMMED CELL-DEATH CONTAINING SUPEROXIDE-DISMUTASE SACCHAROMYCES-CEREVISIAE LIFE-SPAN OXIDATIVE DAMAGE REGULATES APOPTOSIS CYTOCHROME-C PROTEIN STRESS BAX Cell Biology
中文摘要	Apoptosis-inducing factor (AIF) and AIF-homologous mitochondrion-associated inducer of death (AMID) are both mitochondrial flavoproteins that trigger caspase-independent apoptosis. Phylogenetic analysis suggests that these two proteins evolutionarily diverge back from their common prokaryote ancestor. Compared with AIF, the proapoptotic nature of AMID and its mode of action are much less clarified. Here, we show that overexpression of yeast AMID homologue internal NADH dehydrogenase (NDI1), but not external NADH dehydrogenase (NDE1), can cause apoptosis-like cell death, and this effect can be repressed by increased respiration on glucose-limited media. This result indicates that the regulatory network of energy metabolism, in particular the cross-talk between mitochondria and the rest of the cell, is involved in Ndi1p-induced yeast cell apoptosis. The apoptotic effect of NDI1 overexpression is associated with increased production of reactive oxygen species (ROS) in mitochondria. In addition, NDI1 overexpression in sod2 background causes cell lethality in both fermentable and semifermentable media. Interruption of certain components in the electron transport chain can suppress the growth inhibition from Ndi1p overexpression. We finally show that disruption of NDI1 or NDE1 decreases ROS production and elongates the chronological life span of yeast, accompanied by the loss of survival fitness. Implication of these findings for Ndi1p-induced apoptosis is discussed.
语种	英语 ; 英语
出版者	AMER SOC CELL BIOLOGY ; BETHESDA ; 8120 WOODMONT AVE, STE 750, BETHESDA, MD 20814-2755 USA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/26642]
专题	清华大学
推荐引用方式 GB/T 7714	Li, W,Sun, LB,Liang, QL,et al. Yeast AMID homologue Ndi1p displays respiration-restricted apoptotic activity and is involved in chronological aging[J],2010, 2010.
APA	Li, W,Sun, LB,Liang, QL,Wang, J,Mo, WK,&Zhou, B.(2010).Yeast AMID homologue Ndi1p displays respiration-restricted apoptotic activity and is involved in chronological aging..
MLA	Li, W,et al."Yeast AMID homologue Ndi1p displays respiration-restricted apoptotic activity and is involved in chronological aging".(2010).

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