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	Cloning and characterization of an IKK homologue from pearl oyster, Pinctada fucata
	Xiong, Xunhao ; Feng, Qiaoli ; Chen, Lei ; Xie, Liping ; Zhang, Rongqing
	2010-05-11 ; 2010-05-11
关键词	nuclear factor kappa B pathway I kappa B kinase mollusk immunity Pinctado fucata NF-KAPPA-B TUMOR-NECROSIS-FACTOR CRASSOSTREA-GIGAS IN-VITRO CG-TIMP TRANSCRIPTION FACTORS EASTERN OYSTER POTENTIAL ROLE GENE ACTIVATION Immunology Zoology
中文摘要	I kappa B kinase (IKK) play central roles in cell signaling by regulating nuclear factor-kappa B (NF-kappa B) activation, which is involved in inflammatory response, proliferation, development and bone homeostasis. We report here for the first time that an IKK homologue was cloned and functionally characterized in pearl oyster, Pinctada fucata. The full-length cDNA consists of 2546 bp with an ORF encoding a 737 amino acids protein. The putative pearl oyster IKK protein (Pf-IKK) possesses the characteristic organization of the mammalian IKK proteins, namely an amino-terminal kinase domain followed by a leucine zipper region and a carboxylterminal helix-loop-helix motif. Real-time PCR (RT-PCR) analysis indicated that Pf-IKK was ubiquitously expressed in pearl oyster. We also found that lipopotysaccharides (LPS) transiently stimulates I kappa B alpha degradation, but not expression Levels of Pf-IKK. When transfected into NIH3T3 cells, Pf-IKK activated the expression of NF-kappa B-controlled reporter gene and induced NF-kappa B translocation, whereas the activation was greatly deduced by pyrrolidine dithiocarbamate (PDTC). We also found that overexpression of Pf-IKK increased the alkaline phosphatase (ALP) activity significantly. Based on the results and the homology to the vertebrate NF-kappa B cascade, these studies help to highlight a potentially important regulatory pathway to the study of the related functions in mollusks. (C) 2007 Elsevier Ltd. All rights reserved.
语种	英语 ; 英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD ; OXFORD ; THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/26633]
专题	清华大学
推荐引用方式 GB/T 7714	Xiong, Xunhao,Feng, Qiaoli,Chen, Lei,et al. Cloning and characterization of an IKK homologue from pearl oyster, Pinctada fucata[J],2010, 2010.
APA	Xiong, Xunhao,Feng, Qiaoli,Chen, Lei,Xie, Liping,&Zhang, Rongqing.(2010).Cloning and characterization of an IKK homologue from pearl oyster, Pinctada fucata..
MLA	Xiong, Xunhao,et al."Cloning and characterization of an IKK homologue from pearl oyster, Pinctada fucata".(2010).

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