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	Negative dielectrophoretic force assisted construction of ordered neuronal networks on cell positioning bioelectronic chips
	Zhe Yu ; Guangxin Xiang ; Liangbin Pan ; Lihua Huang ; Zhongyao Yu ; Wanli Xing ; Jing Cheng
	2010-05-11 ; 2010-05-11
关键词	Practical Experimental/ bioelectric phenomena biotechnology cellular biophysics electrophoresis elemental semiconductors microelectrodes neural nets neurophysiology silicon/ negative dielectrophoretic force ordered neuronal networks construction cell positioning bioelectronic chips neuron patterning neurological research neuron trapping electrode arrays electrode design electrode fabrication silicon substrate nonuniform electric field electrode size working solution conductivity cell concentration neuron suspension neuron adhesion neuron culture neuron viability neurite development neuronal networks monitoring Si/ A8730C Electrical activity in neurophysiological processes A8725 Cellular biophysics A8245 Electrochemistry and electrophoresis/ Si/sur Si/el
中文摘要	Developing new methods and technologies in order to pattern neurons into regular networks is of utmost scientific interest in the field of neurological research. An efficient method here is developed for trapping neurons and constructing ordered neuronal networks on bioelectronic chips by using arrayed negative dielectrophoretic (DEP) forces. A special bioelectronic chip with well defined positioning electrode arrays was designed and fabricated on silicon substrate. When a high frequency AC signal was applied, the cell positioning bioelectronic chip (CPBC) is able to provide a well-defined nonuniform electric field, and thus generate negative DEP forces. The parameters, such as size of positioning electrode, conductivity of working solution, amplitude and frequency of power signal and cell concentration, were investigated to optimize the performance of the CPBC. When the neuron suspension was added onto the energized bioelectronic chip, the neurons were immediately trapped and quickly formed the predetermined pattern. Neurons may adhere and then be cultured directly on the CPBC, and show good neuron viability and neurite development. The formation of the ordered neuronal networks after two-week culture demonstrates that negative dielectrophoretic force assisted construction of ordered neuronal networks is effective, and it could be used to assist in monitoring functional activities of neuronal networks.
语种	英语 ; 英语
出版者	Kluwer Academic Publishers ; USA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/26631]
专题	清华大学
推荐引用方式 GB/T 7714	Zhe Yu,Guangxin Xiang,Liangbin Pan,et al. Negative dielectrophoretic force assisted construction of ordered neuronal networks on cell positioning bioelectronic chips[J],2010, 2010.
APA	Zhe Yu.,Guangxin Xiang.,Liangbin Pan.,Lihua Huang.,Zhongyao Yu.,...&Jing Cheng.(2010).Negative dielectrophoretic force assisted construction of ordered neuronal networks on cell positioning bioelectronic chips..
MLA	Zhe Yu,et al."Negative dielectrophoretic force assisted construction of ordered neuronal networks on cell positioning bioelectronic chips".(2010).

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