QQ客服 官方微博 反馈留言

	Cloning and characterization of a heterogeneous nuclear ribonucleoprotein homolog from pearl oyster, Pinctada fucata
	Xiong, Xunhao ; Feng, Qiaoli ; Xie, Liping ; Zhang, Rongqing
	2010-05-11 ; 2010-05-11
关键词	RNA-recognition motif heterogeneous nuclear ribonucleoprotein glycine-rich protein Pinctada fucata RNA-BINDING PROTEIN MESSENGER-RNA ARGININE METHYLATION RECOGNITION MOTIF GENE-EXPRESSION SH3 DOMAIN K-PROTEIN HNRNP IDENTIFICATION EMBRYOGENESIS Biochemistry & Molecular Biology Biophysics
中文摘要	Heterogeneous nuclear ribonucleoproteins (hnRNPs) have fundamental roles in the post-transcriptional control of gene expression. Here, a cDNA encoding a presumed full-length RNA-binding protein was isolated from pearl oyster (Pinctada fucata) using reverse transcription-polymerase chain reaction with degenerate primers, and rapid amplification of cDNA ends. The full-length cDNA consists of 2737 bp with an open reading frame encoding a protein of 624 amino acids with a predicted molecular weight of 69 kDa and isoelectric point of 8.7. The putative pearl oyster RNA-binding protein presents a molecular organization close to the hnRNPs, namely an acidic N-terminal followed by three RNA-recognition motifs and a C-terminal that contains RG/RGG repeated motifs. When transfected HeLa cells, the Pf-HRPH (Pinctada fucata hnRNP homolog) gene expression product was found only in nuclei, revealing that it is a nuclear protein. The expression pattern was also investigated by quantitative real-time polymerase chain reaction, indicating that Pf-HRPH mRNA was abundantly expressed in gonad, gill, and viscera. As far as we know, the putative Pf-HRPH is the first hnRNP homolog cloned in mollusks. These data are significant for further study of the multiple functions of RNA-binding protein.
语种	英语 ; 英语
出版者	BLACKWELL PUBLISHING ; OXFORD ; 9600 GARSINGTON RD, OXFORD OX4 2DQ, OXON, ENGLAND
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/26626]
专题	清华大学
推荐引用方式 GB/T 7714	Xiong, Xunhao,Feng, Qiaoli,Xie, Liping,et al. Cloning and characterization of a heterogeneous nuclear ribonucleoprotein homolog from pearl oyster, Pinctada fucata[J],2010, 2010.
APA	Xiong, Xunhao,Feng, Qiaoli,Xie, Liping,&Zhang, Rongqing.(2010).Cloning and characterization of a heterogeneous nuclear ribonucleoprotein homolog from pearl oyster, Pinctada fucata..
MLA	Xiong, Xunhao,et al."Cloning and characterization of a heterogeneous nuclear ribonucleoprotein homolog from pearl oyster, Pinctada fucata".(2010).

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。