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	Effects of insulin-like growth factor 1 on synaptic excitability in cultured rat hippocampal neurons
	Xing, Changhong ; Yin, Yanling ; Chang, Rui ; Gong, Xiaoming ; He, Xiangping ; Xie, Zuoping
	2010-05-11 ; 2010-05-11
关键词	insulin-like growth factor 1 excitatory postsynaptic currents inhibitory postsynaptic currents patch clamp MAPK GROWTH-FACTOR-I ALZHEIMERS-DISEASE RECEPTOR-BINDING IGF-I INSULIN BRAIN EXPRESSION PLASTICITY PATHWAYS CHANNELS Neurosciences
中文摘要	Insulin-like growth factor 1 (IGF-1) has important functions in the brain, including metabolic, neurotrophic, neuromodulatory and neuroendocrine actions, and it also prevents beta amyloid-induced death of hippocampal neurons. However, its functions in the synaptic excitability remain uncertain. Here we investigated the effects of IGF-1 on synaptic excitability in cultured rat hippocampal neurons using whole-cell patch clamp recordings. Incubation the hippocampal neurons with different concentrations of IGF-1 for 24 h or 30 min significantly increased the frequency of spontaneous excitatory postsynaptic currents (sEPSCs), but had no effect on the frequency of miniature EPSCs (mEPSCs) and spontaneous inhibitory postsynaptic currents (sIPSCs). The mean amplitudes, rise, and decay kinetics of sEPSCs, mEPSCs, and sIPSCs were not significantly affected by IGF-1, indicating that IGF-1 increased the probability of neurotransmitter release but did not modulate postsynaptic receptors. The effects of IGF-1 were mediated by mitogen-activated protein kinase (MAPK). IGF-1 activated the ERK1/2 signaling pathway in cultured hippocampal neurons, and the inhibitor PD98059 blocked the enhancement of sEPSCs induced by IGF-1. These results demonstrated the regulatory function of IGF-1 on synaptic excitability in hippocampal neurons and its underlying signaling mechanism. (C) 2007 Elsevier Inc. All rights reserved.
语种	英语 ; 英语
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE ; SAN DIEGO ; 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/26588]
专题	清华大学
推荐引用方式 GB/T 7714	Xing, Changhong,Yin, Yanling,Chang, Rui,et al. Effects of insulin-like growth factor 1 on synaptic excitability in cultured rat hippocampal neurons[J],2010, 2010.
APA	Xing, Changhong,Yin, Yanling,Chang, Rui,Gong, Xiaoming,He, Xiangping,&Xie, Zuoping.(2010).Effects of insulin-like growth factor 1 on synaptic excitability in cultured rat hippocampal neurons..
MLA	Xing, Changhong,et al."Effects of insulin-like growth factor 1 on synaptic excitability in cultured rat hippocampal neurons".(2010).

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