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	Determination of baicalin in rat hippocampus by RP-LC after intravenous administration of flavonoids from Scutellariae radix
	Zhang, LJ ; Xing, DM ; Ding, Y ; Wang, RF ; Du, LJ
	2010-05-11 ; 2010-05-11
关键词	column liquid chromatography baicalin Scutellariae radix hippocampus in rats OREN-GEDOKU-TO TRANSIENT CEREBRAL-ISCHEMIA MICE BAICALENSIS IMPAIRMENT LESIONS TANG Biochemical Research Methods Chemistry, Analytical
中文摘要	A rapid and sensitive method to assay baicalin in rat hippocampus was applied using a simple liquid-liquid extraction technique followed by high-performance liquid chromatography. Baicalin and the internal standard, 4-nitro-benzoic acid, were extracted twice from the homogenized solution with acetonitrile and after centrifugation the combined extracts were evaporated. To the remaining residue 0.1 mL of methanol were added to obtain the sample solution. A 10 mu L volume of sample solution was injected onto HPLC for analysis carried out on a Zorbax SB-C-18 column using a mobile phase of methanol-water-H3PO4 (45:55:0.2, , v/v/v, pH 3.0) at 277 nm with a UV detector. The calibration curve for baicalin was linear over the concentration range of 0.05-1.6 mu g mg(-1) in hippocampus. Recoveries were reasonable for routine analyses (> 88%) and the LOD and LOQ ranged from 0.006 to 0.009 mu g mg(-1) and 0.015 to 0.035 mu g mg(-1), respectively. The coefficient of variation of the assay precision was less than 5.9%, and the accuracy exceeded 98%. The method was applied to determine the time course of baicalin in rat hippocampus, following the intravenously administration of flavonoids from Scutellariae Radix extract at 90 mg kg(-1) of baicalin to a male Wistar rat. This method provides a very simple, sensitive, and accurate way to determine baicalin concentrations in rat hippocampus.
语种	英语 ; 英语
出版者	VIEWEG ; WIESBADEN ; ABRAHAM-LINCOLN-STRABE 46, POSTFACH 15 47, D-65005 WIESBADEN, GERMANY
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/26577]
专题	清华大学
推荐引用方式 GB/T 7714	Zhang, LJ,Xing, DM,Ding, Y,et al. Determination of baicalin in rat hippocampus by RP-LC after intravenous administration of flavonoids from Scutellariae radix[J],2010, 2010.
APA	Zhang, LJ,Xing, DM,Ding, Y,Wang, RF,&Du, LJ.(2010).Determination of baicalin in rat hippocampus by RP-LC after intravenous administration of flavonoids from Scutellariae radix..
MLA	Zhang, LJ,et al."Determination of baicalin in rat hippocampus by RP-LC after intravenous administration of flavonoids from Scutellariae radix".(2010).

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