QQ客服 官方微博 反馈留言

	cDNA cloning and characterization of a novel calmodulin-like protein from pearl oyster Pinctada fucata
	Li, S ; Xie, LP ; Ma, ZJ ; Zhang, RQ
	2010-05-11 ; 2010-05-11 ; OCT
关键词	calmodulin calmodulin-like protein calcium oyster Pinctada fucata CALCIUM-BINDING PROTEINS SODIUM DODECYL-SULFATE PHOSPHORYLATION EXPRESSION GENE IDENTIFICATION CALCIFICATION LOCALIZATION PSEUDOGENE METABOLISM Biochemistry & Molecular Biology
中文摘要	Calcium metabolism in oysters is a very complicated and highly controlled physiological and biochemical process. However, the regulation of calcium metabolism in oyster is poorly understood. Our previous study showed that calmodulin (CaM) seemed to play a regulatory role in the process of oyster calcium metabolism. In this study, a full-length cDNA encoding a novel calmodulin-like protein (CaLP) with a long C-terminal sequence was identified from pearl oyster Pinctada fucata, expressed in Escherichia coli and characterized in vitro. The oyster CaLP mRNA was expressed in all tissues tested, with the highest levels in the mantle that is a key organ involved in calcium secretion. In situ hybridization analysis reveals that CaLP mRNA is expressed strongly in the outer and inner epithelial cells of the inner fold, the outer epithelial cells of the middle fold, and the dorsal region of the mantle. The oyster CaLP protein, with four putative Ca2+-binding domains, is highly heat-stable and has a potentially high affinity for calcium. CaLP also displays typical Ca2+-dependent electrophoretic shift, Ca2+-binding activity and significant Ca2+-induced conformational changes. Ca2+-dependent affinity chromatography analysis demonstrated that oyster CaLP was able to interact with some different target proteins from those of oyster CaM in the mantle and the gill. In summary, our results have demonstrated that the oyster CaLP is a novel member of the CaM superfamily, and suggest that the oyster CaLP protein might play a different role from CaM in the regulation of oyster calcium metabolism.
语种	英语 ; 英语
出版者	BLACKWELL PUBLISHING ; OXFORD ; 9600 GARSINGTON RD, OXFORD OX4 2DQ, OXON, ENGLAND
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/26555]
专题	清华大学
推荐引用方式 GB/T 7714	Li, S,Xie, LP,Ma, ZJ,et al. cDNA cloning and characterization of a novel calmodulin-like protein from pearl oyster Pinctada fucata[J],2010, 2010, OCT.
APA	Li, S,Xie, LP,Ma, ZJ,&Zhang, RQ.(2010).cDNA cloning and characterization of a novel calmodulin-like protein from pearl oyster Pinctada fucata..
MLA	Li, S,et al."cDNA cloning and characterization of a novel calmodulin-like protein from pearl oyster Pinctada fucata".(2010).

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。