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	Oligomerization study of NHR3 and NHR4 domains from ETO protein involved in t(8;21)-associated acute myeloid leukemia
	Wu, DH ; Yang, HT ; Xue, XY ; Liang, WX ; Miao, XY ; Chen, SJ ; Pang, H
	2010-05-11 ; 2010-05-11
关键词	NHR3 domain NHR4 domain tetramer dimer ETO protein AML1-ETO fusion protein FUSION TRANSCRIPT T(8-21) TARGET COMPLEX T(8/21) HEMATOPOIESIS RECRUITMENT REPRESSION PARTNER BINDS Multidisciplinary Sciences
中文摘要	Little had been known about ETO protein until t(8;21) was found in 12%-15% of acute myeloid leukemia which resulted in AML1-ETO fusion protein. ETO protein has four conserved nervy homology regions termed NHR1-4. A lot have already been known about NHR1, 2,4: NHR1 is homologous with the Drosophila TATA-box-associated factor 110 (TAF110); NHR2 is a dimerization domain associated with mSin3A/HDAC; NHR4 is MYND class of zinc ringers associated with NCoR/SMRT/HDAC. Only the function of NHR3 remains unclear. In order to investigate whether NHR3 domain could participate in oligomerization, we cloned and purified this domain. Through gel filtration chromatography, dynamic light scattering and dissolved crystal electrophoresis, we found that NHR3 domain was a tight tetramer. Then we cloned NHR3+4 domain (i.e. NHR3 domain plus NHR4 domain), and discovered, by gel filtration chromatography and native PAGE, that NHR3+4 domain could form dimer in solution. This was the first time to observe that NHR3 and NHR4 domains may have some contribution to the oligomerization of ETO protein, which might recruit corepressors in the form of dimer, and stabilize ETO dimerization through convergent strength of NHR2, NHR3 and NHR4 domains and then stabilize corepressors recruitment. These speculations are very worthy of further evaluation.
语种	英语 ; 英语
出版者	SCIENCE PRESS ; BEIJING ; 16 DONGHUANGCHENGGEN NORTH ST, BEIJING 100717, PEOPLES R CHINA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/26548]
专题	清华大学
推荐引用方式 GB/T 7714	Wu, DH,Yang, HT,Xue, XY,et al. Oligomerization study of NHR3 and NHR4 domains from ETO protein involved in t(8;21)-associated acute myeloid leukemia[J],2010, 2010.
APA	Wu, DH.,Yang, HT.,Xue, XY.,Liang, WX.,Miao, XY.,...&Pang, H.(2010).Oligomerization study of NHR3 and NHR4 domains from ETO protein involved in t(8;21)-associated acute myeloid leukemia..
MLA	Wu, DH,et al."Oligomerization study of NHR3 and NHR4 domains from ETO protein involved in t(8;21)-associated acute myeloid leukemia".(2010).

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