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	Urea-induced inactivation and unfolding of recombinant phospholipid hydroperoxide glutathione peroxidase from Oryza sativa
	Feng, Wang ; Hui-ping, Zhou ; Bao-hua, Kong ; Jing-hua, Fan ; Hai-ru, Chen ; Jin-yuan, Liu
	2010-05-11 ; 2010-05-11
关键词	circular dichroism fluorescence conformational change phospholipid hydroperoxide glutathione peroxidase(PHGPx) urea titration MOLECULAR CHARACTERIZATION CONFORMATIONAL TRANSITION KINETIC MEASUREMENTS SALT-STRESS PROTEIN GENE CLONING PHGPX IDENTIFICATION PURIFICATION Chemistry, Multidisciplinary
中文摘要	Phospholipid hydroperoxide glutathione peroxidase is an antioxidant enzyme that has the highest capability of reducing membrane-bound hydroperoxy lipids as compared to free organic and inorganic hydroperoxides amongst the glutathione peroxidases. In this study, urea-induced effects on the inactivation and unfolding of a recombinant phospholipid hydroperoxide glutathione peroxidase(PHGPx) from Oryza sativa were investigated by means of circular dichroism and fluorescence spectroscopy. With the increase of urea concentration, the residual activity of OsPHGPx decreases. correspondingly. When the urea concentration is above 5. 0 mol/L, there was no residual activity. In addition, the observed changes in intrinsic tryptophan fluorescence, the binding of the hydrophobic fluorescence probe ANS, and the far UV CD describe a common dependence on the concentration of urea suggesting that the conformational features of the native OsPHGPx are lost in a highly cooperative single transition. The unfolding process comprises of three zones: the native base-line zone between 0 and 2. 5 mol/L urea, the transition zone between 2. 5 and 5. 5 mol/L urea, and the denatured base-line zone above 5.5 mol/L urea. The transition zone has a midpoint at about 4. 0 mol/L urea.
语种	英语 ; 英语
出版者	HIGHER EDUCATION PRESS ; BEIJING ; SHATANHOU ST 55, BEIJING 100009, PEOPLES R CHINA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/26517]
专题	清华大学
推荐引用方式 GB/T 7714	Feng, Wang,Hui-ping, Zhou,Bao-hua, Kong,et al. Urea-induced inactivation and unfolding of recombinant phospholipid hydroperoxide glutathione peroxidase from Oryza sativa[J],2010, 2010.
APA	Feng, Wang,Hui-ping, Zhou,Bao-hua, Kong,Jing-hua, Fan,Hai-ru, Chen,&Jin-yuan, Liu.(2010).Urea-induced inactivation and unfolding of recombinant phospholipid hydroperoxide glutathione peroxidase from Oryza sativa..
MLA	Feng, Wang,et al."Urea-induced inactivation and unfolding of recombinant phospholipid hydroperoxide glutathione peroxidase from Oryza sativa".(2010).

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