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	Candidate peptide-vaccines induced immunity against CSFV and identified sequential neutralizing determinants in antigenic domain A of glycoprotein E2
	Dong, XN ; Chen, YH
	2010-05-11 ; 2010-05-11
关键词	classical swine fever virus (CSFV) sequential neutralizing epitope peptide-vaccine (PV) multi-peptide-vaccine (MPV) marker vaccine CLASSICAL SWINE-FEVER HOG-CHOLERA VIRUS PROTECTIVE IMMUNITY PROTEINS EPITOPES ANTIBODIES INDUCTION Immunology Medicine, Research & Experimental Veterinary Sciences
中文摘要	Antigenic domain A is a highly conserved unit on envelope protein E2 of classical swine fever virus (CSFV). It was found that mutant E2 containing only unit A, with the unit BC deleted, provided immunized pigs with complete protection against the lethal challenge. In this study, six overlapping peptides (A1-A6) covering this unit were synthesized and conjugated to bovine serum albumin (BSA). Two candidate multi-peptide-vaccines (MPVs) using aluminum adjuvant successfully induced potent immunity against CSFV in pigs. Although both candidate MPVs failed to provide complete protection, they showed better protective activity than that induced by C-strain. Subsequently, neutralizing epitopes in unit A were identified using a panel of peptide-vaccines (PVs). Six candidate peptide-vaccines (PV-An, n = 1-6) were separately given to six groups of pigs. Among these candidates, PV-A2 and PV-A6 exhibited the most potent protective activity, while the other four showed weaker or almost no effects. Moreover, the polyclonal antibodies induced by PV-A2 and PV-A6 were capable of neutralizing C-strain virus at the dilution 1: 16 in vitro. Thus, two principal sequential neutralizing determinants covered by peptide A2 (aa792-814) and A6 (aa844-865) were demonstrated to exist in the antigenic domain A, and can be recruited in developing new effective "marker vaccine" against CSFV. (c) 2005 Elsevier Ltd. All rights reserved.
语种	英语 ; 英语
出版者	ELSEVIER SCI LTD ; OXFORD ; THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/26503]
专题	清华大学
推荐引用方式 GB/T 7714	Dong, XN,Chen, YH. Candidate peptide-vaccines induced immunity against CSFV and identified sequential neutralizing determinants in antigenic domain A of glycoprotein E2[J],2010, 2010.
APA	Dong, XN,&Chen, YH.(2010).Candidate peptide-vaccines induced immunity against CSFV and identified sequential neutralizing determinants in antigenic domain A of glycoprotein E2..
MLA	Dong, XN,et al."Candidate peptide-vaccines induced immunity against CSFV and identified sequential neutralizing determinants in antigenic domain A of glycoprotein E2".(2010).

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