The design and application of DNA chips for early detection of SARS-CoV from clinical samples | |
Zhou, YM ; Yang, RQ ; Tao, SC ; Li, Z ; Zhang, Q ; Gao, HF ; Zhang, ZW ; Du, JY ; Zhu, PX ; Ren, LL ; Zhang, L ; Wang, D ; Guo, L ; Wang, YB ; Guo, Y ; Zhang, Y ; Zhao, CZ ; Wang, C ; Jiang, D ; Liu, YH ; Yang, HW ; Rong, L ; Zhao, YJ ; An, S ; Li, Z ; Fan, XD ; Wang, JW ; Cheng, Y ; Liu, O ; Zheng, Z ; Zuo, HC ; Shan, QZ ; Ruan, L ; Lu, ZX ; Hung, T ; Cheng, J | |
2010-05-11 ; 2010-05-11 | |
关键词 | SARS coronavirus SARS-CoV early detection gene chip ACUTE RESPIRATORY SYNDROME GENOME SEQUENCE CORONAVIRUS MICROARRAYS Virology |
中文摘要 | Background: SARS coronavirus has been identified as the cause of severe acute respiratory syndrome (SARS). Few tests allow confirmation or exclusion of SARS within the first few days of infection. A gene chip is a useful tool for the study of microbial infections mainly for its capability of performing multi-target analysis in a single test. Objectives: Investigate the possibility of early detection of SARS virus from clinical samples using the gene chip-based method. Study design: We purified RNA from SARS-CoV obtained from routinely collected peripheral blood and sputum samples of 34 patients who had been identified as probable SARS patients by following the interim U.S. case definition. Four segments of the SARS-CoV were amplified using reverse transcription-nested PCR and the products examined using the 70-mer gene chips for SARS-CoV detection. Results: A blind-test of both peripheral blood and sputum specimens lead to the positive detection of SARS-CoV in 31 out of 34 patients. SARS-CoV was not found in peripheral blood or sputum specimens from three patients. Two of the 34 patients were only 3 days post-onset of symptoms and were subsequently confirmed to be SARS positive. Our results indicate that the gene chip-based molecular test is specific for SARS-CoV and allows early detection of patients with SARS with detection rate about 8% higher than the single PCR test when the sputum sample is available. (C) 2004 Elsevier B.V. All rights reserved. |
语种 | 英语 ; 英语 |
出版者 | ELSEVIER SCIENCE BV ; AMSTERDAM ; PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS |
内容类型 | 期刊论文 |
源URL | [http://hdl.handle.net/123456789/26462] ![]() |
专题 | 清华大学 |
推荐引用方式 GB/T 7714 | Zhou, YM,Yang, RQ,Tao, SC,et al. The design and application of DNA chips for early detection of SARS-CoV from clinical samples[J],2010, 2010. |
APA | Zhou, YM.,Yang, RQ.,Tao, SC.,Li, Z.,Zhang, Q.,...&Cheng, J.(2010).The design and application of DNA chips for early detection of SARS-CoV from clinical samples.. |
MLA | Zhou, YM,et al."The design and application of DNA chips for early detection of SARS-CoV from clinical samples".(2010). |
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