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	A protein A-based immobilization stategy for love wave immunosensor
	Liu Hong ; Gan Zhixian ; Wang Yuansheng ; Liu Qingshan ; Zhu Huizhong
	2010-05-10 ; 2010-05-10
关键词	love wave immunosensor staphylococcal enterotoxins B protein A SENSOR Chemistry, Analytical
中文摘要	A Love wave immunosensor based on protein A immobilization strategy for staphylococcal enterotoxins B (SEB) is presented. The Love wave devices with the substrate of ST-cut quartz, IDTs and the guiding layers of SiO2 were fabricated successfully. In the immobilization of antibodies to the sensor by protein A-based immobilization procedure, NaBH4, a selective reducing agent was used to reduce the Schiff's bases resulting from the reaction of glutaraldehyde-activated surface with protein amino groups to improve the regeneration of the immunosensor. The active area of sensor was precoated with gamma-aminopropylethoxysilane (APTES) and glutaraldehyde, protein A were coupled to the surface by formation of Schiff's base and reduction with 100 mmol/L NaBH4 to form stable -CH2-NH- bonds, and antibody molecule probes were immobilized in a highly oriented manner by the protein A on SiO2 surface. Results indicated that such a preparation can greatly increase regeneration and sensibility of the immunosensor, up to 5 successive assay repetitions to 1.0 mg/L SEB were achieved without significant loss of sensitivity using the new immunosensor, moreover, SEB serums concentration as low as 1.0 mu g/L, could be detected by the same system. The surface morphology of the active area characterized by using atomic force microscopy revealed that in contrast to the glutaraldehyde immobilization, protein A-NaBH, procedure could allow antibody probes bound with higher bioactivity and loading amount, achieving better SEB-binding capabilities.
语种	中文 ; 中文
出版者	SCIENCE CHINA PRESS ; BEIJING ; 16 DONGHUANGCHENGGEN NORTH ST, BEIJING 100717, PEOPLES R CHINA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/24840]
专题	清华大学
推荐引用方式 GB/T 7714	Liu Hong,Gan Zhixian,Wang Yuansheng,et al. A protein A-based immobilization stategy for love wave immunosensor[J],2010, 2010.
APA	Liu Hong,Gan Zhixian,Wang Yuansheng,Liu Qingshan,&Zhu Huizhong.(2010).A protein A-based immobilization stategy for love wave immunosensor..
MLA	Liu Hong,et al."A protein A-based immobilization stategy for love wave immunosensor".(2010).

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