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	Collagen I gel can facilitate homogenous bone formation of adipose-derived stem cells in PLGA-beta-TCP scaffold
	Hao, Wei ; Hu, Yun-Yu ; Wei, Yi-Yong ; Pang, Long ; Lv, Rong ; Bai, Jian-Ping ; Xiong, Zhuo ; Jiang, Ming
	2010-05-10 ; 2010-05-10
关键词	adipose-derived stem cells collagen I gel PLGA-beta-TCP scaffold osteogenic differentiation homogenous bone formation TISSUE-ENGINEERED BONE STROMAL CELLS ARTICULAR-CARTILAGE CALVARIAL DEFECTS CRANIAL DEFECTS HUMAN MARROW VIVO REGENERATION REPAIR MATRIX Anatomy & Morphology Cell Biology Developmental Biology
中文摘要	Cell-based tissue engineering is thought to be a new therapy for treatment of bone defects and nonunions after trauma and tumor resection. In this study, we explore the in vitro and in vivo osteogenesis of a novel biomimetic construct fabricated by using collagen I gel to suspend rabbit adipose-derived stem cells (rASCs) into a porous poly(lactic-coglycolic) acid-beta-tricalcium phosphate (PLGA-beta-TCP) scaffold (rASCs-COL/PLGA-beta-TCP). In vitro and in vivo studies of the rASCs-COL/PLGA-beta-TCP composite (group A) were carried out compared with the single combination of rASCs and PLGA-beta-TCP (rASCs/PLGA-beta-TCP; group B), the combination of acellular collagen I gel and PLGA-beta-TCP (COL/PLGA-beta-TCP; group C), and the PLGA-beta-TCP scaffold (group D). Composites of different groups were cultured in vitro for 2 weeks in osteogenic medium and then implanted into the autologous muscular intervals for 8 weeks. After 2 weeks of in vitro culture, alkaline phosphatase activity and extracellular matrix mineralization in group A were significantly higher than in group B (p < 0.01, n = 4). In vivo osteogenesis was evaluated by radiographic and histological analyses. The calcification level was radiographically evident in group A, whereas no apparent calcification was observed in groups B, C and D (n = 4). In group A, woven bone with a trabecular structure was formed, while in group B, only osteoid tissue was observed. Meanwhile, the bone-forming area in group A was significantly higher than in group B (p < 0.01, n = 4). No bone formation was observed in groups C or D (n = 4). In conclusion, by using collagen I gel to suspend rASCs into porous PLGA-beta-TCP scaffold, osteogenic differentiation of rASCs can be improved and homogeneous bone tissue can be successfully formed in vivo. Copyright (C) 2007 S. Karger AG, Basel.
语种	英语 ; 英语
出版者	KARGER ; BASEL ; ALLSCHWILERSTRASSE 10, CH-4009 BASEL, SWITZERLAND
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/24100]
专题	清华大学
推荐引用方式 GB/T 7714	Hao, Wei,Hu, Yun-Yu,Wei, Yi-Yong,et al. Collagen I gel can facilitate homogenous bone formation of adipose-derived stem cells in PLGA-beta-TCP scaffold[J],2010, 2010.
APA	Hao, Wei.,Hu, Yun-Yu.,Wei, Yi-Yong.,Pang, Long.,Lv, Rong.,...&Jiang, Ming.(2010).Collagen I gel can facilitate homogenous bone formation of adipose-derived stem cells in PLGA-beta-TCP scaffold..
MLA	Hao, Wei,et al."Collagen I gel can facilitate homogenous bone formation of adipose-derived stem cells in PLGA-beta-TCP scaffold".(2010).

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