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	Inducement and degradation function of p450 in Phanerochaete chrysospofium by in situ spectroscopic analysis
	Da-Liang, Ning ; Hui, Wang ; Yuan, Zhuang ; Dong, Li
	2010-05-10 ; 2010-05-10
关键词	reduced carbon monooxide binding difference spectrum Phanerochaete chrysosporium p450 inducement degradation CATALYZING HYDROXYLATION XENOBIOTIC INDUCTION BIOTRANSFORMATION PENTACHLOROPHENOL NAPHTHALENE EXPRESSION HERBICIDES PRODUCTS BENZOATE CULTURES Chemistry, Multidisciplinary
中文摘要	The method of inducement-in situ spectra analysis was developed and used to directly detect and characterize microsome P450 and its inducement in Phanerochaete chrysosporium(PC) by n-hexane, benzoic acid, naphthalene and pentachlorophenol at molecular level. The results demonstrate that the content of P450 after inducement by n-hexane, benzoic acid, naphthalene reached a high level of 7.5, 5.6 and 3.1 times of that without inducement, respectively. The inducement of P450 in fungi by pentachlorophenol has not been reported before this study. The substrate binding spectra of benzoic acid with P450 front PC grown in both low nitrogen medium and PDB medium, and NADPH-dependent degradation of benzoic acid in PC microsome were detected, which provide a direct evidence of the function of P450 to catabolize benzoic acid. However, the inhibition was not observed during the degradation of benzoic acid by PC with P450 inhibitor aminobenzotriazole, which indicates that other catabolic pathway may also exist in PC.
语种	中文 ; 中文
出版者	HIGHER EDUCATION PRESS ; BEIJING ; SHATANHOU ST 55, BEIJING 100009, PEOPLES R CHINA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/23434]
专题	清华大学
推荐引用方式 GB/T 7714	Da-Liang, Ning,Hui, Wang,Yuan, Zhuang,et al. Inducement and degradation function of p450 in Phanerochaete chrysospofium by in situ spectroscopic analysis[J],2010, 2010.
APA	Da-Liang, Ning,Hui, Wang,Yuan, Zhuang,&Dong, Li.(2010).Inducement and degradation function of p450 in Phanerochaete chrysospofium by in situ spectroscopic analysis..
MLA	Da-Liang, Ning,et al."Inducement and degradation function of p450 in Phanerochaete chrysospofium by in situ spectroscopic analysis".(2010).

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