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	A highly specific immunoassay for microcystin-LR detection based on a monoclonal antibody
	Sheng, Jian-Wu ; He, Miao ; Shi, Han-Chang
	2010-05-10 ; 2010-05-10
关键词	microcystin-LR monoclonal antibody MC8C10 enzyme-linked immunosorbent assay (ELISA) high specificity PHOSPHATASE INHIBITION ASSAY CYANOBACTERIAL TOXINS POLYCLONAL ANTIBODIES WATER NODULARIN Chemistry, Analytical
中文摘要	Microcystins (MC) are cyanobacterial hepatotoxins responsible for animal-poisoning and human health incidents. Immunoassays provide a sensitive and fast means to detect these toxins, but cross-reactivity (CR) characteristic of different antibodies was variable. Here, we have produced and characterized a monoclonal antibody (Clone MC8C10) with highly specificity against the most frequent and most toxic variant of microcystins, MC-LR. MC8C10 is more specific against MC-LR among the reported antibodies before. The immunogen was synthesized from the modified MC-LR and bovine serum albumin (BSA). An indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) with MC8C10 was established to detect the MCs in waters, which showed highly specificity with MC-LR, and have a detection limit for MC-LR 0.1 mu g L-1, the 50% inhibition concentration (IC50) for MC-LR was 1.8 +/- 0.1 mu g L-1 and the quantitative detection range was from 0.3 to 10 mu g L-1. The [4-arginine] microcystins and the nodularin-R showed lower cross-reactivates (CR < 10%), and other MCs such as MC-LF and MC-LW are not recognized (CR < 10(-4)). The analysis results of real water samples with ic-ELISA showed that all the coefficients of variation were less than 15%, and the recovery was (100.3 +/- 5.9)%. So the highly specific ic-ELISA will commendably suit for sensitive analysis for MC-LR in surface water as well as drinking water. (C) 2007 Elsevier B.V. All rights reserved.
语种	英语 ; 英语
出版者	ELSEVIER SCIENCE BV ; AMSTERDAM ; PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/23366]
专题	清华大学
推荐引用方式 GB/T 7714	Sheng, Jian-Wu,He, Miao,Shi, Han-Chang. A highly specific immunoassay for microcystin-LR detection based on a monoclonal antibody[J],2010, 2010.
APA	Sheng, Jian-Wu,He, Miao,&Shi, Han-Chang.(2010).A highly specific immunoassay for microcystin-LR detection based on a monoclonal antibody..
MLA	Sheng, Jian-Wu,et al."A highly specific immunoassay for microcystin-LR detection based on a monoclonal antibody".(2010).

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